Microbial removal of Atmospheric carbon tetrachloride in bulk aerobic soils

Y. Mendoza, K. D. Goodwin, James D Happell

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Atmospheric concentrations of carbon tetrachloride (CCl 4) were removed by bulk aerobic soils from tropical, subtropical, and boreal environments. Removal was observed in all tested soil types, indicating that the process was widespread. The flux measured in field chamber experiments was 0.24 ± 0.10 nmol CCl 4 (m 2 day) -1 (average ± standard deviation [SD]; n = 282). Removal of CCl 4 and removal of methane (CH 4 ) were compared to explore whether the two processes were linked. Removal of both gases was halted in laboratory samples that were autoclaved, dry heated, or incubated in the presence of mercuric chloride (HgCl 2). In marl soils, treatment with antibiotics such as tetracycline and streptomycin caused partial inhibition of CCl 4 (50%) and CH 4 (76%) removal, but removal was not affected in soils treated with nystatin or myxothiazol. These data indicated that bacteria contributed to the soil removal of CCl 4 and that microeukaryotes may not have played a significant role. Amendments of methanol, acetate, and succinate to soil samples enhanced CCl 4 removal by 59%, 293%, and 72%, respectively. Additions of a variety of inhibitors and substrates indicated that nitrification, methanogenesis, or biological reduction of nitrate, nitrous oxide, or sulfate (e.g., occurring in possible anoxic microzones) did not play a significant role in the removal of CCl 4. Methyl fluoride inhibited removal of CH 4 but not CCl 4, indicating that CH 4 and CCl 4 removals were not directly linked. Furthermore, CCl 4 removal was not affected in soils amended with copper sulfate or methane, supporting the results with MeF and suggesting that the observed CCl 4 removal was not significantly mediated by methanotrophs.

Original languageEnglish (US)
Pages (from-to)5835-5841
Number of pages7
JournalApplied and Environmental Microbiology
Volume77
Issue number17
DOIs
StatePublished - Sep 1 2011

Fingerprint

carbon tetrachloride
Carbon Tetrachloride
Soil
carbon
methane
soil
nystatin
marl
methanotrophs
mercuric chloride
Methane
copper sulfate
nitrate reduction
succinic acid
fluorides
streptomycin
methane production
nitrous oxide
tetracycline
soil treatment

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Microbial removal of Atmospheric carbon tetrachloride in bulk aerobic soils. / Mendoza, Y.; Goodwin, K. D.; Happell, James D.

In: Applied and Environmental Microbiology, Vol. 77, No. 17, 01.09.2011, p. 5835-5841.

Research output: Contribution to journalArticle

@article{d2ee10f47fc341c59db32857f8ba31fc,
title = "Microbial removal of Atmospheric carbon tetrachloride in bulk aerobic soils",
abstract = "Atmospheric concentrations of carbon tetrachloride (CCl 4) were removed by bulk aerobic soils from tropical, subtropical, and boreal environments. Removal was observed in all tested soil types, indicating that the process was widespread. The flux measured in field chamber experiments was 0.24 ± 0.10 nmol CCl 4 (m 2 day) -1 (average ± standard deviation [SD]; n = 282). Removal of CCl 4 and removal of methane (CH 4 ) were compared to explore whether the two processes were linked. Removal of both gases was halted in laboratory samples that were autoclaved, dry heated, or incubated in the presence of mercuric chloride (HgCl 2). In marl soils, treatment with antibiotics such as tetracycline and streptomycin caused partial inhibition of CCl 4 (50{\%}) and CH 4 (76{\%}) removal, but removal was not affected in soils treated with nystatin or myxothiazol. These data indicated that bacteria contributed to the soil removal of CCl 4 and that microeukaryotes may not have played a significant role. Amendments of methanol, acetate, and succinate to soil samples enhanced CCl 4 removal by 59{\%}, 293{\%}, and 72{\%}, respectively. Additions of a variety of inhibitors and substrates indicated that nitrification, methanogenesis, or biological reduction of nitrate, nitrous oxide, or sulfate (e.g., occurring in possible anoxic microzones) did not play a significant role in the removal of CCl 4. Methyl fluoride inhibited removal of CH 4 but not CCl 4, indicating that CH 4 and CCl 4 removals were not directly linked. Furthermore, CCl 4 removal was not affected in soils amended with copper sulfate or methane, supporting the results with MeF and suggesting that the observed CCl 4 removal was not significantly mediated by methanotrophs.",
author = "Y. Mendoza and Goodwin, {K. D.} and Happell, {James D}",
year = "2011",
month = "9",
day = "1",
doi = "10.1128/AEM.05341-11",
language = "English (US)",
volume = "77",
pages = "5835--5841",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "17",

}

TY - JOUR

T1 - Microbial removal of Atmospheric carbon tetrachloride in bulk aerobic soils

AU - Mendoza, Y.

AU - Goodwin, K. D.

AU - Happell, James D

PY - 2011/9/1

Y1 - 2011/9/1

N2 - Atmospheric concentrations of carbon tetrachloride (CCl 4) were removed by bulk aerobic soils from tropical, subtropical, and boreal environments. Removal was observed in all tested soil types, indicating that the process was widespread. The flux measured in field chamber experiments was 0.24 ± 0.10 nmol CCl 4 (m 2 day) -1 (average ± standard deviation [SD]; n = 282). Removal of CCl 4 and removal of methane (CH 4 ) were compared to explore whether the two processes were linked. Removal of both gases was halted in laboratory samples that were autoclaved, dry heated, or incubated in the presence of mercuric chloride (HgCl 2). In marl soils, treatment with antibiotics such as tetracycline and streptomycin caused partial inhibition of CCl 4 (50%) and CH 4 (76%) removal, but removal was not affected in soils treated with nystatin or myxothiazol. These data indicated that bacteria contributed to the soil removal of CCl 4 and that microeukaryotes may not have played a significant role. Amendments of methanol, acetate, and succinate to soil samples enhanced CCl 4 removal by 59%, 293%, and 72%, respectively. Additions of a variety of inhibitors and substrates indicated that nitrification, methanogenesis, or biological reduction of nitrate, nitrous oxide, or sulfate (e.g., occurring in possible anoxic microzones) did not play a significant role in the removal of CCl 4. Methyl fluoride inhibited removal of CH 4 but not CCl 4, indicating that CH 4 and CCl 4 removals were not directly linked. Furthermore, CCl 4 removal was not affected in soils amended with copper sulfate or methane, supporting the results with MeF and suggesting that the observed CCl 4 removal was not significantly mediated by methanotrophs.

AB - Atmospheric concentrations of carbon tetrachloride (CCl 4) were removed by bulk aerobic soils from tropical, subtropical, and boreal environments. Removal was observed in all tested soil types, indicating that the process was widespread. The flux measured in field chamber experiments was 0.24 ± 0.10 nmol CCl 4 (m 2 day) -1 (average ± standard deviation [SD]; n = 282). Removal of CCl 4 and removal of methane (CH 4 ) were compared to explore whether the two processes were linked. Removal of both gases was halted in laboratory samples that were autoclaved, dry heated, or incubated in the presence of mercuric chloride (HgCl 2). In marl soils, treatment with antibiotics such as tetracycline and streptomycin caused partial inhibition of CCl 4 (50%) and CH 4 (76%) removal, but removal was not affected in soils treated with nystatin or myxothiazol. These data indicated that bacteria contributed to the soil removal of CCl 4 and that microeukaryotes may not have played a significant role. Amendments of methanol, acetate, and succinate to soil samples enhanced CCl 4 removal by 59%, 293%, and 72%, respectively. Additions of a variety of inhibitors and substrates indicated that nitrification, methanogenesis, or biological reduction of nitrate, nitrous oxide, or sulfate (e.g., occurring in possible anoxic microzones) did not play a significant role in the removal of CCl 4. Methyl fluoride inhibited removal of CH 4 but not CCl 4, indicating that CH 4 and CCl 4 removals were not directly linked. Furthermore, CCl 4 removal was not affected in soils amended with copper sulfate or methane, supporting the results with MeF and suggesting that the observed CCl 4 removal was not significantly mediated by methanotrophs.

UR - http://www.scopus.com/inward/record.url?scp=80052701055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052701055&partnerID=8YFLogxK

U2 - 10.1128/AEM.05341-11

DO - 10.1128/AEM.05341-11

M3 - Article

C2 - 21724884

AN - SCOPUS:80052701055

VL - 77

SP - 5835

EP - 5841

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 17

ER -