Microarray analysis of hippocampal CA1 neurons implicates early endosomal dysfunction during Alzheimer's disease progression

Stephen D. Ginsberg, Melissa J. Alldred, Scott E. Counts, Anne M. Cataldo, Rachael L. Neve, Ying Jiang, Joanne Wuu, Moses V. Chao, Elliott J. Mufson, Ralph A. Nixon, Shaoli Che

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Background: Endocytic dysfunction and neurotrophin signaling deficits may underlie the selective vulnerability of hippocampal neurons during the progression of Alzheimer's disease (AD), although there is little direct in vivo and biochemical evidence to support this hypothesis. Methods: Microarray analysis of hippocampal CA1 pyramidal neurons acquired via laser capture microdissection was performed using postmortem brain tissue. Validation was achieved using real-time quantitative polymerase chain reaction and immunoblot analysis. Mechanistic studies were performed using human fibroblasts subjected to overexpression with viral vectors or knockdown via small interference RNA. Results: Expression levels of genes regulating early endosomes (rab5) and late endosomes (rab7) are selectively upregulated in homogeneous populations of CA1 neurons from individuals with mild cognitive impairment and AD. The levels of these genes are selectively increased as antemortem measures of cognition decline during AD progression. Hippocampal quantitative polymerase chain reaction and immunoblot analyses confirmed increased levels of these transcripts and their respective protein products. Elevation of select rab GTPases regulating endocytosis paralleled the downregulation of genes encoding the neurotrophin receptors TrkB and TrkC. Overexpression of rab5 in cells suppressed TrkB expression, whereas knockdown of TrkB expression did not alter rab5 levels, suggesting that TrkB downregulation is a consequence of endosomal dysfunction associated with elevated rab5 levels in early AD. Conclusions: These data support the hypothesis that neuronal endosomal dysfunction is associated with preclinical AD. Increased endocytic pathway activity, driven by elevated rab GTPase expression, may result in long-term deficits in hippocampal neurotrophic signaling and represent a key pathogenic mechanism underlying AD progression.

Original languageEnglish
Pages (from-to)885-893
Number of pages9
JournalBiological Psychiatry
Volume68
Issue number10
DOIs
StatePublished - Nov 15 2010
Externally publishedYes

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Microarray Analysis
Disease Progression
Alzheimer Disease
Neurons
rab GTP-Binding Proteins
Endosomes
Down-Regulation
Laser Capture Microdissection
Nerve Growth Factor Receptors
Pyramidal Cells
Nerve Growth Factors
RNA Interference
Endocytosis
Cognition
Genes
Real-Time Polymerase Chain Reaction
Fibroblasts
Gene Expression
Polymerase Chain Reaction
Brain

Keywords

  • Laser capture microdissection
  • mild cognitive impairment
  • qPCR
  • rab5
  • rab7
  • RNA amplification
  • siRNA
  • TrkB

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

Ginsberg, S. D., Alldred, M. J., Counts, S. E., Cataldo, A. M., Neve, R. L., Jiang, Y., ... Che, S. (2010). Microarray analysis of hippocampal CA1 neurons implicates early endosomal dysfunction during Alzheimer's disease progression. Biological Psychiatry, 68(10), 885-893. https://doi.org/10.1016/j.biopsych.2010.05.030

Microarray analysis of hippocampal CA1 neurons implicates early endosomal dysfunction during Alzheimer's disease progression. / Ginsberg, Stephen D.; Alldred, Melissa J.; Counts, Scott E.; Cataldo, Anne M.; Neve, Rachael L.; Jiang, Ying; Wuu, Joanne; Chao, Moses V.; Mufson, Elliott J.; Nixon, Ralph A.; Che, Shaoli.

In: Biological Psychiatry, Vol. 68, No. 10, 15.11.2010, p. 885-893.

Research output: Contribution to journalArticle

Ginsberg, SD, Alldred, MJ, Counts, SE, Cataldo, AM, Neve, RL, Jiang, Y, Wuu, J, Chao, MV, Mufson, EJ, Nixon, RA & Che, S 2010, 'Microarray analysis of hippocampal CA1 neurons implicates early endosomal dysfunction during Alzheimer's disease progression', Biological Psychiatry, vol. 68, no. 10, pp. 885-893. https://doi.org/10.1016/j.biopsych.2010.05.030
Ginsberg, Stephen D. ; Alldred, Melissa J. ; Counts, Scott E. ; Cataldo, Anne M. ; Neve, Rachael L. ; Jiang, Ying ; Wuu, Joanne ; Chao, Moses V. ; Mufson, Elliott J. ; Nixon, Ralph A. ; Che, Shaoli. / Microarray analysis of hippocampal CA1 neurons implicates early endosomal dysfunction during Alzheimer's disease progression. In: Biological Psychiatry. 2010 ; Vol. 68, No. 10. pp. 885-893.
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abstract = "Background: Endocytic dysfunction and neurotrophin signaling deficits may underlie the selective vulnerability of hippocampal neurons during the progression of Alzheimer's disease (AD), although there is little direct in vivo and biochemical evidence to support this hypothesis. Methods: Microarray analysis of hippocampal CA1 pyramidal neurons acquired via laser capture microdissection was performed using postmortem brain tissue. Validation was achieved using real-time quantitative polymerase chain reaction and immunoblot analysis. Mechanistic studies were performed using human fibroblasts subjected to overexpression with viral vectors or knockdown via small interference RNA. Results: Expression levels of genes regulating early endosomes (rab5) and late endosomes (rab7) are selectively upregulated in homogeneous populations of CA1 neurons from individuals with mild cognitive impairment and AD. The levels of these genes are selectively increased as antemortem measures of cognition decline during AD progression. Hippocampal quantitative polymerase chain reaction and immunoblot analyses confirmed increased levels of these transcripts and their respective protein products. Elevation of select rab GTPases regulating endocytosis paralleled the downregulation of genes encoding the neurotrophin receptors TrkB and TrkC. Overexpression of rab5 in cells suppressed TrkB expression, whereas knockdown of TrkB expression did not alter rab5 levels, suggesting that TrkB downregulation is a consequence of endosomal dysfunction associated with elevated rab5 levels in early AD. Conclusions: These data support the hypothesis that neuronal endosomal dysfunction is associated with preclinical AD. Increased endocytic pathway activity, driven by elevated rab GTPase expression, may result in long-term deficits in hippocampal neurotrophic signaling and represent a key pathogenic mechanism underlying AD progression.",
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AU - Ginsberg, Stephen D.

AU - Alldred, Melissa J.

AU - Counts, Scott E.

AU - Cataldo, Anne M.

AU - Neve, Rachael L.

AU - Jiang, Ying

AU - Wuu, Joanne

AU - Chao, Moses V.

AU - Mufson, Elliott J.

AU - Nixon, Ralph A.

AU - Che, Shaoli

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N2 - Background: Endocytic dysfunction and neurotrophin signaling deficits may underlie the selective vulnerability of hippocampal neurons during the progression of Alzheimer's disease (AD), although there is little direct in vivo and biochemical evidence to support this hypothesis. Methods: Microarray analysis of hippocampal CA1 pyramidal neurons acquired via laser capture microdissection was performed using postmortem brain tissue. Validation was achieved using real-time quantitative polymerase chain reaction and immunoblot analysis. Mechanistic studies were performed using human fibroblasts subjected to overexpression with viral vectors or knockdown via small interference RNA. Results: Expression levels of genes regulating early endosomes (rab5) and late endosomes (rab7) are selectively upregulated in homogeneous populations of CA1 neurons from individuals with mild cognitive impairment and AD. The levels of these genes are selectively increased as antemortem measures of cognition decline during AD progression. Hippocampal quantitative polymerase chain reaction and immunoblot analyses confirmed increased levels of these transcripts and their respective protein products. Elevation of select rab GTPases regulating endocytosis paralleled the downregulation of genes encoding the neurotrophin receptors TrkB and TrkC. Overexpression of rab5 in cells suppressed TrkB expression, whereas knockdown of TrkB expression did not alter rab5 levels, suggesting that TrkB downregulation is a consequence of endosomal dysfunction associated with elevated rab5 levels in early AD. Conclusions: These data support the hypothesis that neuronal endosomal dysfunction is associated with preclinical AD. Increased endocytic pathway activity, driven by elevated rab GTPase expression, may result in long-term deficits in hippocampal neurotrophic signaling and represent a key pathogenic mechanism underlying AD progression.

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