TY - JOUR
T1 - Microarray analysis of gene expression in adult retinal ganglion cells
AU - Ivanov, Dmitry
AU - Dvoriantchikova, Galina
AU - Nathanson, Lubov
AU - McKinnon, Stuart J.
AU - Shestopalov, Valery I.
N1 - Funding Information:
We thank Jeffrey Goldberg for the gift of T11D7e2 cells and the advice in RGCs immunopurification, Vlad Slepak, Sara Cleveland for the manuscript discussion and the staff of the University of Miami Microarray Core Facility for the expert assistance. Grant support: NIH R01-EY14232 (V.S.), NIH R01-EY16516 (S.M.), NIH Center Grant P30 EY014801 , Fight for Sight fellowship PD05034 (D.I.), Inc., a Research to Prevent Blindness (RPB) Career Development Award (V.S.) and an unrestricted grant to the University of Miami, and Duke University Medical Center.
PY - 2006/1/9
Y1 - 2006/1/9
N2 - Retinal ganglion cells (RGCs) transfer visual information to the brain and are known to be susceptible to selective degeneration in various neuropathies such as glaucoma. This selective vulnerability suggests that these highly specialized neurons possess a distinct gene expression profile that becomes altered by neuropathy-associated stresses, which lead to the RGC death. In this study, to identify genes expressed predominantly in adult RGCs, a global transcriptional profile of purified primary RGCs has been compared to that of the whole retina. To avoid alterations of the original gene expression profile by cell culture conditions, we isolated RNA directly from adult RGCs purified by immunopanning without prior sub-cultivation. Genes expressed predominantly in RGCs included: Nrg1, Rgn, 14-3-3 family (Ywhah, Ywhaz, Ywhab), Nrn1, Gap43, Vsnl1, Rgs4. Some of these genes may serve as novel markers for these neurons. Our analysis revealed enrichment in genes controlling the pro-survival pathways in RGCs as compared to other retinal cells.
AB - Retinal ganglion cells (RGCs) transfer visual information to the brain and are known to be susceptible to selective degeneration in various neuropathies such as glaucoma. This selective vulnerability suggests that these highly specialized neurons possess a distinct gene expression profile that becomes altered by neuropathy-associated stresses, which lead to the RGC death. In this study, to identify genes expressed predominantly in adult RGCs, a global transcriptional profile of purified primary RGCs has been compared to that of the whole retina. To avoid alterations of the original gene expression profile by cell culture conditions, we isolated RNA directly from adult RGCs purified by immunopanning without prior sub-cultivation. Genes expressed predominantly in RGCs included: Nrg1, Rgn, 14-3-3 family (Ywhah, Ywhaz, Ywhab), Nrn1, Gap43, Vsnl1, Rgs4. Some of these genes may serve as novel markers for these neurons. Our analysis revealed enrichment in genes controlling the pro-survival pathways in RGCs as compared to other retinal cells.
KW - Cell markers
KW - Gene expression
KW - Immunopanning
KW - Retina
KW - Retinal ganglion cells
KW - RNA amplification
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U2 - 10.1016/j.febslet.2005.12.017
DO - 10.1016/j.febslet.2005.12.017
M3 - Article
C2 - 16376886
AN - SCOPUS:29344454910
VL - 580
SP - 331
EP - 335
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -