Abstract
Retinal ganglion cells (RGCs) transfer visual information to the brain and are known to be susceptible to selective degeneration in various neuropathies such as glaucoma. This selective vulnerability suggests that these highly specialized neurons possess a distinct gene expression profile that becomes altered by neuropathy-associated stresses, which lead to the RGC death. In this study, to identify genes expressed predominantly in adult RGCs, a global transcriptional profile of purified primary RGCs has been compared to that of the whole retina. To avoid alterations of the original gene expression profile by cell culture conditions, we isolated RNA directly from adult RGCs purified by immunopanning without prior sub-cultivation. Genes expressed predominantly in RGCs included: Nrg1, Rgn, 14-3-3 family (Ywhah, Ywhaz, Ywhab), Nrn1, Gap43, Vsnl1, Rgs4. Some of these genes may serve as novel markers for these neurons. Our analysis revealed enrichment in genes controlling the pro-survival pathways in RGCs as compared to other retinal cells.
| Original language | English |
|---|---|
| Pages (from-to) | 331-335 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 580 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 9 2006 |
Fingerprint
Keywords
- Cell markers
- Gene expression
- Immunopanning
- Retina
- Retinal ganglion cells
- RNA amplification
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Microarray analysis of gene expression in adult retinal ganglion cells. / Ivanov, Dmitry V; Dvoriantchikova, Galina; Nathanson, Lubov; McKinnon, Stuart J.; Shestopalov, Valery I.
In: FEBS Letters, Vol. 580, No. 1, 09.01.2006, p. 331-335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microarray analysis of gene expression in adult retinal ganglion cells
AU - Ivanov, Dmitry V
AU - Dvoriantchikova, Galina
AU - Nathanson, Lubov
AU - McKinnon, Stuart J.
AU - Shestopalov, Valery I
PY - 2006/1/9
Y1 - 2006/1/9
N2 - Retinal ganglion cells (RGCs) transfer visual information to the brain and are known to be susceptible to selective degeneration in various neuropathies such as glaucoma. This selective vulnerability suggests that these highly specialized neurons possess a distinct gene expression profile that becomes altered by neuropathy-associated stresses, which lead to the RGC death. In this study, to identify genes expressed predominantly in adult RGCs, a global transcriptional profile of purified primary RGCs has been compared to that of the whole retina. To avoid alterations of the original gene expression profile by cell culture conditions, we isolated RNA directly from adult RGCs purified by immunopanning without prior sub-cultivation. Genes expressed predominantly in RGCs included: Nrg1, Rgn, 14-3-3 family (Ywhah, Ywhaz, Ywhab), Nrn1, Gap43, Vsnl1, Rgs4. Some of these genes may serve as novel markers for these neurons. Our analysis revealed enrichment in genes controlling the pro-survival pathways in RGCs as compared to other retinal cells.
AB - Retinal ganglion cells (RGCs) transfer visual information to the brain and are known to be susceptible to selective degeneration in various neuropathies such as glaucoma. This selective vulnerability suggests that these highly specialized neurons possess a distinct gene expression profile that becomes altered by neuropathy-associated stresses, which lead to the RGC death. In this study, to identify genes expressed predominantly in adult RGCs, a global transcriptional profile of purified primary RGCs has been compared to that of the whole retina. To avoid alterations of the original gene expression profile by cell culture conditions, we isolated RNA directly from adult RGCs purified by immunopanning without prior sub-cultivation. Genes expressed predominantly in RGCs included: Nrg1, Rgn, 14-3-3 family (Ywhah, Ywhaz, Ywhab), Nrn1, Gap43, Vsnl1, Rgs4. Some of these genes may serve as novel markers for these neurons. Our analysis revealed enrichment in genes controlling the pro-survival pathways in RGCs as compared to other retinal cells.
KW - Cell markers
KW - Gene expression
KW - Immunopanning
KW - Retina
KW - Retinal ganglion cells
KW - RNA amplification
UR - http://www.scopus.com/inward/record.url?scp=29344454910&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=29344454910&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2005.12.017
DO - 10.1016/j.febslet.2005.12.017
M3 - Article
C2 - 16376886
AN - SCOPUS:29344454910
VL - 580
SP - 331
EP - 335
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -