Microarray analysis of gene expression in adult retinal ganglion cells

Dmitry V Ivanov, Galina Dvoriantchikova, Lubov Nathanson, Stuart J. McKinnon, Valery I Shestopalov

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Retinal ganglion cells (RGCs) transfer visual information to the brain and are known to be susceptible to selective degeneration in various neuropathies such as glaucoma. This selective vulnerability suggests that these highly specialized neurons possess a distinct gene expression profile that becomes altered by neuropathy-associated stresses, which lead to the RGC death. In this study, to identify genes expressed predominantly in adult RGCs, a global transcriptional profile of purified primary RGCs has been compared to that of the whole retina. To avoid alterations of the original gene expression profile by cell culture conditions, we isolated RNA directly from adult RGCs purified by immunopanning without prior sub-cultivation. Genes expressed predominantly in RGCs included: Nrg1, Rgn, 14-3-3 family (Ywhah, Ywhaz, Ywhab), Nrn1, Gap43, Vsnl1, Rgs4. Some of these genes may serve as novel markers for these neurons. Our analysis revealed enrichment in genes controlling the pro-survival pathways in RGCs as compared to other retinal cells.

Original languageEnglish
Pages (from-to)331-335
Number of pages5
JournalFEBS Letters
Volume580
Issue number1
DOIs
StatePublished - Jan 9 2006

Fingerprint

Retinal Ganglion Cells
Microarray Analysis
Microarrays
Gene expression
Genes
Gene Expression
Neurons
Transcriptome
Cell death
Cell culture
Brain
Cells
RNA
Glaucoma
Retina
Cell Death
Cell Culture Techniques

Keywords

  • Cell markers
  • Gene expression
  • Immunopanning
  • Retina
  • Retinal ganglion cells
  • RNA amplification

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Microarray analysis of gene expression in adult retinal ganglion cells. / Ivanov, Dmitry V; Dvoriantchikova, Galina; Nathanson, Lubov; McKinnon, Stuart J.; Shestopalov, Valery I.

In: FEBS Letters, Vol. 580, No. 1, 09.01.2006, p. 331-335.

Research output: Contribution to journalArticle

Ivanov, Dmitry V ; Dvoriantchikova, Galina ; Nathanson, Lubov ; McKinnon, Stuart J. ; Shestopalov, Valery I. / Microarray analysis of gene expression in adult retinal ganglion cells. In: FEBS Letters. 2006 ; Vol. 580, No. 1. pp. 331-335.
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