Microarray analysis of fiber cell maturation in the lens

Dmitry Ivanov, Galina Dvoriantchikova, Anna Pestova, Lubov Nathanson, Valery I. Shestopalov

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The mammalian lens consists of an aged core of quiescent cells enveloped by layers of mature fully elongated cells and younger, continuously elongating transcriptionally active cells. The fiber cell maturation is initiated when fiber cells cease to elongate. The process of maturation represents a radical switch from active elongation to a life-long quiescence and has not been studied previously. It may also include critical stages of preparation for the organelle removal and denucleation. In the present study, we used laser capture microdisection (LCM) microdissection and RNA amplification to compare global gene expression profiles of young elongating and mature, non-elongating fiber cells. Analysis of microarray data from three independent dye-swap experiments identified 65 differentially expressed genes (FDR < 0.1) with greater than 2-fold change in expression levels. Microarray array results for a group of randomly selected genes were confirmed by quantitative RT-PCR. These microarray results provide clues to understanding the molecular pathways underlying lens development. The identified changes in the profile of gene expression reflected a shift in cell physiology characterizing the lens fiber maturation.

Original languageEnglish (US)
Pages (from-to)1213-1219
Number of pages7
JournalFEBS letters
Volume579
Issue number5
DOIs
StatePublished - Feb 14 2005

Keywords

  • Differentiation
  • Gene expression profiling
  • Laser capture microdissection
  • Lens
  • Microarray
  • RNA amplification

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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