Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation

A. I. Pogue, J. G. Cui, Y. Y. Li, Y. Zhao, F. Culicchia, W. J. Lukiw

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Micro RNAs (miRNAs) are post-transcriptional modulators of gene expression that regulate the stability and translation of their target messenger RNAs (mRNAs). Here we report that the levels of a human brain-enriched miRNA-125b are up-regulated in interleukin-6 (IL-6)-stressed normal human astrocytes (NHA), a treatment known to induce astrogliosis. In vitro, anti-miRNA-125b added exogenously to IL-6-stressed NHA cultures attenuated both glial cell proliferation and increased the expression of the cyclin-dependent kinase inhibitor 2A (CDKN2A), a miRNA-125b target and negative regulator of cell growth. A strong positive correlation between miRNA-125b abundance and the glial cell markers glial fibrillary acidic protein (GFAP) and vimentin, and CDKN2A down-regulation was noted in advanced Alzheimer's disease (AD) and in Down's syndrome (DS) brain, chronic neurological disorders associated with astrogliosis. The results suggest that miRNA-125b up-regulation contributes to astrogliosis and to defects in the cell cycle that are characteristic of degenerating brain tissues.

Original languageEnglish
Pages (from-to)18-22
Number of pages5
JournalNeuroscience Letters
Volume476
Issue number1
DOIs
StatePublished - May 1 2010

Fingerprint

MicroRNAs
Neuroglia
Cell Proliferation
Cyclin-Dependent Kinase Inhibitor p16
Astrocytes
Interleukin-6
Brain
Glial Fibrillary Acidic Protein
Vimentin
Down Syndrome
Nervous System Diseases
Cell Cycle
Alzheimer Disease
Up-Regulation
Down-Regulation
Gene Expression
Messenger RNA
Growth

Keywords

  • Alzheimer's disease (AD)
  • Astrogliosis
  • CDKN2A
  • Cell-cycle
  • Down's syndrome (DS)
  • GFAP
  • IL-6
  • MiRNA-125b
  • Vimentin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pogue, A. I., Cui, J. G., Li, Y. Y., Zhao, Y., Culicchia, F., & Lukiw, W. J. (2010). Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation. Neuroscience Letters, 476(1), 18-22. https://doi.org/10.1016/j.neulet.2010.03.054

Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation. / Pogue, A. I.; Cui, J. G.; Li, Y. Y.; Zhao, Y.; Culicchia, F.; Lukiw, W. J.

In: Neuroscience Letters, Vol. 476, No. 1, 01.05.2010, p. 18-22.

Research output: Contribution to journalArticle

Pogue, AI, Cui, JG, Li, YY, Zhao, Y, Culicchia, F & Lukiw, WJ 2010, 'Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation', Neuroscience Letters, vol. 476, no. 1, pp. 18-22. https://doi.org/10.1016/j.neulet.2010.03.054
Pogue, A. I. ; Cui, J. G. ; Li, Y. Y. ; Zhao, Y. ; Culicchia, F. ; Lukiw, W. J. / Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation. In: Neuroscience Letters. 2010 ; Vol. 476, No. 1. pp. 18-22.
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