Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation

A. I. Pogue, J. G. Cui, Y. Y. Li, Y. Zhao, F. Culicchia, W. J. Lukiw

Research output: Contribution to journalArticle

105 Scopus citations

Abstract

Micro RNAs (miRNAs) are post-transcriptional modulators of gene expression that regulate the stability and translation of their target messenger RNAs (mRNAs). Here we report that the levels of a human brain-enriched miRNA-125b are up-regulated in interleukin-6 (IL-6)-stressed normal human astrocytes (NHA), a treatment known to induce astrogliosis. In vitro, anti-miRNA-125b added exogenously to IL-6-stressed NHA cultures attenuated both glial cell proliferation and increased the expression of the cyclin-dependent kinase inhibitor 2A (CDKN2A), a miRNA-125b target and negative regulator of cell growth. A strong positive correlation between miRNA-125b abundance and the glial cell markers glial fibrillary acidic protein (GFAP) and vimentin, and CDKN2A down-regulation was noted in advanced Alzheimer's disease (AD) and in Down's syndrome (DS) brain, chronic neurological disorders associated with astrogliosis. The results suggest that miRNA-125b up-regulation contributes to astrogliosis and to defects in the cell cycle that are characteristic of degenerating brain tissues.

Original languageEnglish (US)
Pages (from-to)18-22
Number of pages5
JournalNeuroscience Letters
Volume476
Issue number1
DOIs
StatePublished - May 1 2010

Keywords

  • Alzheimer's disease (AD)
  • Astrogliosis
  • CDKN2A
  • Cell-cycle
  • Down's syndrome (DS)
  • GFAP
  • IL-6
  • MiRNA-125b
  • Vimentin

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation'. Together they form a unique fingerprint.

  • Cite this

    Pogue, A. I., Cui, J. G., Li, Y. Y., Zhao, Y., Culicchia, F., & Lukiw, W. J. (2010). Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation. Neuroscience Letters, 476(1), 18-22. https://doi.org/10.1016/j.neulet.2010.03.054