Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation

A. I. Pogue, J. G. Cui, Y. Y. Li, Y. Zhao, F. Culicchia, W. J. Lukiw

Research output: Contribution to journalArticlepeer-review

112 Scopus citations


Micro RNAs (miRNAs) are post-transcriptional modulators of gene expression that regulate the stability and translation of their target messenger RNAs (mRNAs). Here we report that the levels of a human brain-enriched miRNA-125b are up-regulated in interleukin-6 (IL-6)-stressed normal human astrocytes (NHA), a treatment known to induce astrogliosis. In vitro, anti-miRNA-125b added exogenously to IL-6-stressed NHA cultures attenuated both glial cell proliferation and increased the expression of the cyclin-dependent kinase inhibitor 2A (CDKN2A), a miRNA-125b target and negative regulator of cell growth. A strong positive correlation between miRNA-125b abundance and the glial cell markers glial fibrillary acidic protein (GFAP) and vimentin, and CDKN2A down-regulation was noted in advanced Alzheimer's disease (AD) and in Down's syndrome (DS) brain, chronic neurological disorders associated with astrogliosis. The results suggest that miRNA-125b up-regulation contributes to astrogliosis and to defects in the cell cycle that are characteristic of degenerating brain tissues.

Original languageEnglish (US)
Pages (from-to)18-22
Number of pages5
JournalNeuroscience Letters
Issue number1
StatePublished - May 1 2010


  • Alzheimer's disease (AD)
  • Astrogliosis
  • CDKN2A
  • Cell-cycle
  • Down's syndrome (DS)
  • GFAP
  • IL-6
  • MiRNA-125b
  • Vimentin

ASJC Scopus subject areas

  • Neuroscience(all)


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