Mice lacking NT-3, and its receptor TrkC, exhibit profound deficiencies in CNS glial cells

M. A. Kahn, S. Kumar, Daniel J Liebl, R. Chang, L. F. Parada, Jean De Vellis

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

Neurotrophin-3 (NT-3) and its receptor TrkC are known to be important for neuronal survival. More recently, NT-3 has been implicated as playing a role in oligodendrocyte (OL) proliferation and survival in vitro. Examination of NT-3 and TrkC knockout mice revealed a reduction in NT-3-dependent neurons. To date, no study has examined alterations in glial cell populations in these knockout mice. In this report, we demonstrate a decline in OL progenitor cell numbers within the CNS of NT-3 and TrkC knockout mice. We also observed that immature and mature OL-specific markers were attenuated in the NT-3 and TrkC knockout animals. Deficiencies in other CNS glial cells, including astrocytes and ameboid microglia, were also observed. The subventricular zone (SVZ), a highly proliferative region for progenitor glial cells, was reduced in size. Furthermore, a nuclear-specific stain revealed a decline in the numbers of pyknotic nuclei in and around the SVZ of the knockout mice. These data will support an in vivo NT-3-dependent mechanism for the normal development of CNS glial cells.

Original languageEnglish
Pages (from-to)153-165
Number of pages13
JournalGLIA
Volume26
Issue number2
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

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Keywords

  • Astrocytes
  • CNS
  • Glia
  • Microglia
  • Neurotrophin-3
  • Oligodendrocytes
  • TrkC

ASJC Scopus subject areas

  • Immunology

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