Mice lacking COX10 in skeletal muscle recapitulate the phenotype of progressive mitochondrial myopathies associated with cytochrome c oxidase deficiency

Francisca Diaz, Christine K Thomas, Sofia Garcia, Dayami Hernandez, Carlos T Moraes

Research output: Contribution to journalArticle

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Abstract

We have created a mouse model with an isolated cytochrome c oxidase (COX) deficiency by disrupting the COX10 gene in skeletal muscle. Missense mutations in COX10 have been previously associated with mitochondrial disorders. Cox10p is a protoheme:heme-O-farnesyl transferase required for the synthesis of heme a, the prosthetic group of the catalytic center of COX. COX10 conditional knockout mice were generated by crossing a LoxP-tagged COX10 mouse with a transgenic mouse expressing cre recombinase under the myosin light chain 1f promoter. The COX10 knockout mice were healthy until approximately 3 months of age when they started developing a slowly progressive myopathy. Surprisingly, even though COX activity in COX10 KO muscles was <5% of control muscle at 2.5 months, these muscles were still able to contract at 80-100% of control maximal forces and showed only a 10% increase in fatigability, and no signs of oxidative damage or apoptosis were detected. However, the myopathy worsened with time, particularly in female animals. This COX10 KO mouse allowed us to correlate the muscle function with residual COX activity, an estimate that can help predict the progression pattern of human mitochondrial myopathies.

Original languageEnglish
Pages (from-to)2737-2748
Number of pages12
JournalHuman Molecular Genetics
Volume14
Issue number18
DOIs
StatePublished - Sep 15 2005

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Cytochrome-c Oxidase Deficiency
Mitochondrial Myopathies
Skeletal Muscle
Phenotype
Muscles
Oxidoreductases
Muscular Diseases
Knockout Mice
Mitochondrial Diseases
Myosin Light Chains
Missense Mutation
Transferases
Heme
Transgenic Mice
Apoptosis
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Mice lacking COX10 in skeletal muscle recapitulate the phenotype of progressive mitochondrial myopathies associated with cytochrome c oxidase deficiency. / Diaz, Francisca; Thomas, Christine K; Garcia, Sofia; Hernandez, Dayami; Moraes, Carlos T.

In: Human Molecular Genetics, Vol. 14, No. 18, 15.09.2005, p. 2737-2748.

Research output: Contribution to journalArticle

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