Methylprednisolone and interleukin-10 reduce gray matter damage in the contused Fischer rat thoracic spinal cord but do not improve functional outcome

Toshihiro Takami, Martin Oudega, John R. Bethea, Patrick Wood, Naomi Kleitman, Mary B Bunge

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The effects of two antiinflammatory and neuroprotective agents, methylprednisolone (MP) and interleukin-10 (IL-10), singly and in combination on tissue damage, axonal preservation and functional recovery were studied in the contused adult Fischer rat thoracic spinal cord 12 weeks after injury. MP (30 mg/kg at 5 min, and 2 and 4 h after injury) was administered intravenously and IL-10 (15 or 30 μg/kg at 30 min after injury), intraperitoneally. MP, IL-10, or the combination significantly reduced the volume of damaged tissue (including cavities) compared to control animals. The loss of spinal tissue (cavities) was reduced after treatment with MP alone or combined with IL-10, but not with IL-10 alone. The reduction in tissue damage was confined to spinal gray matter; at the level of the lesion epicenter, the thickness of the lateral white matter columns was similar in all groups. Retrograde tracing using fast blue revealed that the number of spared propriospinal and supraspinal projections was similar in all groups at 12 weeks after the contusion. The open-field BBB-test showed no significant difference in hindlimb locomotion between groups. Our results demonstrate that all tested antiinflammatory treatments significantly increase the volume of spared spinal gray matter 3 months after a moderate contusion of the Fischer rat thoracic spinal cord, but none of the treatments improved axonal preservation or functional recovery.

Original languageEnglish
Pages (from-to)653-666
Number of pages14
JournalJournal of Neurotrauma
Volume19
Issue number5
StatePublished - Jun 13 2002

Fingerprint

Contusions
Inbred F344 Rats
Methylprednisolone
Interleukin-10
Spinal Cord
Thorax
Wounds and Injuries
Anti-Inflammatory Agents
Interleukin-15
Neuroprotective Agents
Locomotion
Hindlimb
Gray Matter

Keywords

  • Cytokine
  • Glucocorticosteroid
  • Inflammation
  • Methylprednisolone sodium succinate
  • Secondary tissue loss
  • Spinal cord injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Methylprednisolone and interleukin-10 reduce gray matter damage in the contused Fischer rat thoracic spinal cord but do not improve functional outcome. / Takami, Toshihiro; Oudega, Martin; Bethea, John R.; Wood, Patrick; Kleitman, Naomi; Bunge, Mary B.

In: Journal of Neurotrauma, Vol. 19, No. 5, 13.06.2002, p. 653-666.

Research output: Contribution to journalArticle

Takami, Toshihiro ; Oudega, Martin ; Bethea, John R. ; Wood, Patrick ; Kleitman, Naomi ; Bunge, Mary B. / Methylprednisolone and interleukin-10 reduce gray matter damage in the contused Fischer rat thoracic spinal cord but do not improve functional outcome. In: Journal of Neurotrauma. 2002 ; Vol. 19, No. 5. pp. 653-666.
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