Methylation state of the prostate specific membrane antigen (PSMA) CpG island in prostate cancer cell lines

Kenneth R. Noss, Rakesh Singal, Sidney R. Grimes

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: PSMA expression varies among prostate cell lines. We examined the role of CpG methylation and histone deacetylation in PSMA transcriptional repression in prostate cell lines. Materials and Methods: The methylation status of a PSMA CpG island was investigated in LNCaP, DU145 and PC3 prostate cell lines. Cells were treated with a demethylating agent and a histone deacetylase inhibitor to determine if PSMA transcription could be activated in nonexpressing cells. A transfection assay with methylated and unmethylated PSMA promoter/enhancer-driven luciferase expression constructs was performed to examine the effect of methylation on transcription. Results: The PSMA CpG island was only methylated in DU145 cells but transcription could not be activated by demethylation or histone deacetylase inhibition. Methylation repressed PSMA transcription in LNCaP cells. Conclusion: Although promoter methylation represses PSMA transcription in LNCaP cells, another method inhibits PSMA expression in DU145 and PC3 cells.

Original languageEnglish
Pages (from-to)1505-1511
Number of pages7
JournalAnticancer Research
Volume22
Issue number3
StatePublished - Aug 14 2002
Externally publishedYes

Fingerprint

CpG Islands
Methylation
Prostatic Neoplasms
Cell Line
Prostate
human glutamate carboxypeptidase II
Histone Deacetylase Inhibitors
Histone Deacetylases
Luciferases
Histones
Transfection

Keywords

  • DNA methylation
  • DU145
  • Gene regulation
  • LNCaP
  • PC3
  • Prostate cancer
  • PSMA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Methylation state of the prostate specific membrane antigen (PSMA) CpG island in prostate cancer cell lines. / Noss, Kenneth R.; Singal, Rakesh; Grimes, Sidney R.

In: Anticancer Research, Vol. 22, No. 3, 14.08.2002, p. 1505-1511.

Research output: Contribution to journalArticle

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N2 - Background: PSMA expression varies among prostate cell lines. We examined the role of CpG methylation and histone deacetylation in PSMA transcriptional repression in prostate cell lines. Materials and Methods: The methylation status of a PSMA CpG island was investigated in LNCaP, DU145 and PC3 prostate cell lines. Cells were treated with a demethylating agent and a histone deacetylase inhibitor to determine if PSMA transcription could be activated in nonexpressing cells. A transfection assay with methylated and unmethylated PSMA promoter/enhancer-driven luciferase expression constructs was performed to examine the effect of methylation on transcription. Results: The PSMA CpG island was only methylated in DU145 cells but transcription could not be activated by demethylation or histone deacetylase inhibition. Methylation repressed PSMA transcription in LNCaP cells. Conclusion: Although promoter methylation represses PSMA transcription in LNCaP cells, another method inhibits PSMA expression in DU145 and PC3 cells.

AB - Background: PSMA expression varies among prostate cell lines. We examined the role of CpG methylation and histone deacetylation in PSMA transcriptional repression in prostate cell lines. Materials and Methods: The methylation status of a PSMA CpG island was investigated in LNCaP, DU145 and PC3 prostate cell lines. Cells were treated with a demethylating agent and a histone deacetylase inhibitor to determine if PSMA transcription could be activated in nonexpressing cells. A transfection assay with methylated and unmethylated PSMA promoter/enhancer-driven luciferase expression constructs was performed to examine the effect of methylation on transcription. Results: The PSMA CpG island was only methylated in DU145 cells but transcription could not be activated by demethylation or histone deacetylase inhibition. Methylation repressed PSMA transcription in LNCaP cells. Conclusion: Although promoter methylation represses PSMA transcription in LNCaP cells, another method inhibits PSMA expression in DU145 and PC3 cells.

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