Methods for fractionation and scintillation counting of radioisotope-labeled polyacrylamide gels

Samuel Ward, David L. Wilson, James J. Gilliam

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

This paper describes two polyacrylamide gel fractionators. One can be easily constructed from disposable syringes and used for fractionating standard-size analytical gels. The second is more complex but can be used for fractionating miniature gels. A rapid method of preparing gel fractions for liquid-scintillation counting is also presented. The method gives high counting efficiency, does not require hydrolysis of the gel prior to counting, and allows gel fractions to be collected directly in scintillation vials.

Original languageEnglish (US)
Pages (from-to)90-97
Number of pages8
JournalAnalytical Biochemistry
Volume38
Issue number1
DOIs
StatePublished - Nov 1970

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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