Methodological aspects of time-course measurements of 14C fixation in marine phytoplankton

Gary L. Hitchcock

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A series of time-course studies were made in conjunction with productivity measurements to determine: (1) the time required for purging sorbed H14CO3- from glass fiber filters, (2) the duration of linearity in H14CO3- uptake in several oceanic environments, and (3) the time required for an "equilibrium" to be established among solvent-extractable and particulate fractions of natural phytoplankton populations. Acidification of sorbed H14CO3- on Whatman GF F filters requires a 60-min period before the sorbed label is reduced to background levels. Time course measurements of H14CO3- fixation in three regions of the northwestern Atlantic (two warm-core rings and the Sargasso Sea) indicated that carbon uptake was linear for at least 4-6 h. The distribution of 14C among various soluble and particulate fractions of labelled natural phytoplankton populations showed that an "equilibrium" was achieved among the various fractions after 1 to 2 h of incubation.

Original languageEnglish (US)
Pages (from-to)233-243
Number of pages11
JournalJournal of Experimental Marine Biology and Ecology
Volume95
Issue number3
DOIs
StatePublished - 1986

Keywords

  • phytoplankton productivity
  • productivity methodology
  • time-course studies

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

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