Methicillin-resistant Staphylococcus aureus susceptibility testing by an automated system, autobac I

Timothy Cleary, D. Maurer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The Autobac I system was used to evaluate the antibiotic susceptibility pattern of methicillin-resistant Staphylococcus aureus isolates. The results of the Autobac I were compared with the results of the disk diffusion method. The disk diffusion susceptibility pattern showed resistance to methicillin/oxacillin, penicillin, erythromycin, clindamycin, and kanamycin. All isolates were susceptible to cephalothin, chloramphenicol, tetracycline, and gentamicin. There was at least 96% agreement using the Autobac I system with all antibiotics except methicillin and clindamycin. Seventy percent of 57 isolates were recorded as susceptible to methicillin, whereas 9% had an intermediate susceptibility. With clindamycin, 14% were recorded as susceptible and 7% were recorded as intermediate. Upon prolonged incubation of the Autobac I cuvette, the agreement between the two methods was 44% for methicillin and 93% for clindamycin. Changes in the environmental conditions, such as use of 5% sodium chloride broth and a 32°C incubation temperature, did not increase the detection of methicillin-resistant isolates by the Autobac I system.

Original languageEnglish
Pages (from-to)837-841
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume13
Issue number5
StatePublished - Dec 1 1978

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Clindamycin
Methicillin-Resistant Staphylococcus aureus
Methicillin
Methicillin Resistance
Anti-Bacterial Agents
Cephalothin
Oxacillin
Kanamycin
Chloramphenicol
Erythromycin
Gentamicins
Tetracycline
Sodium Chloride
Penicillins
Temperature

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Methicillin-resistant Staphylococcus aureus susceptibility testing by an automated system, autobac I. / Cleary, Timothy; Maurer, D.

In: Antimicrobial Agents and Chemotherapy, Vol. 13, No. 5, 01.12.1978, p. 837-841.

Research output: Contribution to journalArticle

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