Abstract
The biochemical effects of acute and chronic psychological stress have been investigated in male Sprague-Dawley rats using a combination of 1H NMR spectral analysis of plasma and conventional hematological analyses. Animals were subjected to 35 consecutive days of 6-h sessions of stress, and following a 9 day break, were stressed for a further 6-h period. Plasma samples were collected at 0, 1, 3, and 6 h on days 1, 9, 21, 35, and 44, measured using 600 MHz 1H NMR spectroscopy, and analyzed by Principal Components Analysis. Time-dependent biochemical effects of psychological stress on a range of endogenous metabolites were evident and were correlated with the intensity of the stress response as defined by corticosterone and hematological parameters. Following acute stress, increases in the levels of glucose and ketone bodies, and decreases in the levels of acetate, alanine, isoleucine, lactate, leucine, valine, and lipoproteins, were observed. Chronic stress-induced increases in plasma levels of alanine, lactate (day 9), and leucine, valine, and choline (day 44) and decreases in acetate (day 9) and lipoprotein concentrations were observed. Positive correlations between plasma corticosterone level and glucose and glycerol, and between plasma lipoprotein concentrations and hemoglobin levels, were established using Projection to Latent Structures (PLS) analysis. This study indicates the potential of using NMR-based metabonomic strategies for the characterization of endogenous metabolic perturbations induced by psychological stressors and lifestyle choices.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2080-2093 |
| Number of pages | 14 |
| Journal | Journal of Proteome Research |
| Volume | 6 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 2007 |
| Externally published | Yes |
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Keywords
- Acute stress
- Allostasis
- Chronic stress
- Glucocorticoid
- Hematology
- Metabonomics
- Metabotype
- Multivariate analysis
- NMR
- Plasma
ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)
Cite this
Metabonomic studies on the physiological effects of acute and chronic psychological stress in sprague-dawley rats. / Teague, Claire R.; Dhabhar, Firdaus; Barton, Richard H.; Beckwith-Hall, Bridgette; Powell, Jonathan; Cobain, Mark; Singer, Burton; McEwen, Bruce S.; Lindon, John C.; Nicholson, Jeremy K.; Holmes, Elaine.
In: Journal of Proteome Research, Vol. 6, No. 6, 01.06.2007, p. 2080-2093.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metabonomic studies on the physiological effects of acute and chronic psychological stress in sprague-dawley rats
AU - Teague, Claire R.
AU - Dhabhar, Firdaus
AU - Barton, Richard H.
AU - Beckwith-Hall, Bridgette
AU - Powell, Jonathan
AU - Cobain, Mark
AU - Singer, Burton
AU - McEwen, Bruce S.
AU - Lindon, John C.
AU - Nicholson, Jeremy K.
AU - Holmes, Elaine
PY - 2007/6/1
Y1 - 2007/6/1
N2 - The biochemical effects of acute and chronic psychological stress have been investigated in male Sprague-Dawley rats using a combination of 1H NMR spectral analysis of plasma and conventional hematological analyses. Animals were subjected to 35 consecutive days of 6-h sessions of stress, and following a 9 day break, were stressed for a further 6-h period. Plasma samples were collected at 0, 1, 3, and 6 h on days 1, 9, 21, 35, and 44, measured using 600 MHz 1H NMR spectroscopy, and analyzed by Principal Components Analysis. Time-dependent biochemical effects of psychological stress on a range of endogenous metabolites were evident and were correlated with the intensity of the stress response as defined by corticosterone and hematological parameters. Following acute stress, increases in the levels of glucose and ketone bodies, and decreases in the levels of acetate, alanine, isoleucine, lactate, leucine, valine, and lipoproteins, were observed. Chronic stress-induced increases in plasma levels of alanine, lactate (day 9), and leucine, valine, and choline (day 44) and decreases in acetate (day 9) and lipoprotein concentrations were observed. Positive correlations between plasma corticosterone level and glucose and glycerol, and between plasma lipoprotein concentrations and hemoglobin levels, were established using Projection to Latent Structures (PLS) analysis. This study indicates the potential of using NMR-based metabonomic strategies for the characterization of endogenous metabolic perturbations induced by psychological stressors and lifestyle choices.
AB - The biochemical effects of acute and chronic psychological stress have been investigated in male Sprague-Dawley rats using a combination of 1H NMR spectral analysis of plasma and conventional hematological analyses. Animals were subjected to 35 consecutive days of 6-h sessions of stress, and following a 9 day break, were stressed for a further 6-h period. Plasma samples were collected at 0, 1, 3, and 6 h on days 1, 9, 21, 35, and 44, measured using 600 MHz 1H NMR spectroscopy, and analyzed by Principal Components Analysis. Time-dependent biochemical effects of psychological stress on a range of endogenous metabolites were evident and were correlated with the intensity of the stress response as defined by corticosterone and hematological parameters. Following acute stress, increases in the levels of glucose and ketone bodies, and decreases in the levels of acetate, alanine, isoleucine, lactate, leucine, valine, and lipoproteins, were observed. Chronic stress-induced increases in plasma levels of alanine, lactate (day 9), and leucine, valine, and choline (day 44) and decreases in acetate (day 9) and lipoprotein concentrations were observed. Positive correlations between plasma corticosterone level and glucose and glycerol, and between plasma lipoprotein concentrations and hemoglobin levels, were established using Projection to Latent Structures (PLS) analysis. This study indicates the potential of using NMR-based metabonomic strategies for the characterization of endogenous metabolic perturbations induced by psychological stressors and lifestyle choices.
KW - Acute stress
KW - Allostasis
KW - Chronic stress
KW - Glucocorticoid
KW - Hematology
KW - Metabonomics
KW - Metabotype
KW - Multivariate analysis
KW - NMR
KW - Plasma
UR - http://www.scopus.com/inward/record.url?scp=34250854091&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250854091&partnerID=8YFLogxK
U2 - 10.1021/pr060412s
DO - 10.1021/pr060412s
M3 - Article
C2 - 17474765
AN - SCOPUS:34250854091
VL - 6
SP - 2080
EP - 2093
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 6
ER -