Metabolomics: Applications and promise in mycobacterial disease

Mehdi Mirsaeidi, Mohammad Mehdi Banoei, Brent W. Winston, Dean E. Schraufnagel

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Until recently, the study of mycobacterial diseases was trapped in culture-based technology that is more than a century old. The use of nucleic acid amplification is changing this, and powerful new technologies are on the horizon. Metabolomics, which is the study of sets of metabolites of both the bacteria and host, is being used to clarify mechanisms of disease, and can identify changes leading to better diagnosis, treatment, and prognostication of mycobacterial diseases. Metabolomic profiles are arrays of biochemical products of genes in their environment. These complex patterns are biomarkers that can allow a more complete understanding of cell function, dysfunction, and perturbation than genomics or proteomics. Metabolomics could herald sweeping advances in personalized medicine and clinical trial design, but the challenges in metabolomics are also great. Measured metabolite concentrations vary with the timing within a condition, the intrinsic biology, the instruments, and the sample preparation. Metabolism profoundly changes with age, sex, variations in gut microbial flora, and lifestyle. Validation of biomarkers is complicated by measurement accuracy, selectivity, linearity, reproducibility, robustness, and limits of detection. The statistical challenges include analysis, interpretation, and description of the vast amount of data generated. Despite these drawbacks, metabolomics provides great opportunity and the potential to understand and manage mycobacterial diseases.

Original languageEnglish (US)
Pages (from-to)1278-1287
Number of pages10
JournalAnnals of the American Thoracic Society
Volume12
Issue number9
DOIs
StatePublished - Sep 1 2015

Keywords

  • Metabolomics
  • Nontuberculous
  • Omics
  • Tuberculosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Fingerprint Dive into the research topics of 'Metabolomics: Applications and promise in mycobacterial disease'. Together they form a unique fingerprint.

  • Cite this