Experiments were designed to study the effect of two pyrogens, a Pseudomonas aeruginosa polysaccharide and a Shigella toxin, upon glycolysis, respiration, and nucleic acid synthesis by normal bone marrow cells and circulating leukemic leukocytes. Both the Pseudomonas polysaccharide and Shigella toxin enhanced glycolysis and oxygen uptake by leukemic leukocytes. The Pseudomonas polysaccharide was the more effective. There was also a suggestive increase in the uptake of C14 adenine into the ribose nucleic acid (RNA) fraction of leukemic leukocytes when high concentrations of Shigella toxin were used. But in lower concentrations Shigella toxin had no effect on the incorporation of C14 adenine into the RNA and desoxyribose nucleic acid of rabbit bone marrow, and neither pyrogen influenced the incorporation of orotic acid into nucleic acids. From the data obtained it may be concluded that the toxic effects of bacterial pyrogens upon leukocytes devolve primarily on metabolic processes other than those concerned with nucleic acid synthesis.
|Original language||English (US)|
|Number of pages||9|
|Journal||The Journal of Laboratory and Clinical Medicine|
|State||Published - Apr 1 1960|
ASJC Scopus subject areas
- Pathology and Forensic Medicine