Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene

Sanjoy K Bhattacharya, Ashok K. Dubey

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Metabolic burden as a consequence of overexpression of target gene in a recombinant strain of E. coli 1727 has been analyzed with respect to maintenance energy coefficient (m). The values of 'm' for the host, uninduced recombinant and IPTG induced recombinant were determined to be 0.12, 0.17 and 0.32 g.g-1.h-1 respectively. Transient plasmid instability and nearly 33% fall in maximum specific growth rate were observed under conditions of enhanced requirements for maintenance energy.

Original languageEnglish
Pages (from-to)1155-1160
Number of pages6
JournalBiotechnology Letters
Volume17
Issue number11
DOIs
StatePublished - Nov 1 1995
Externally publishedYes

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Escherichia coli
Genes
Maintenance
Isopropyl Thiogalactoside
Plasmids
Growth

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology

Cite this

Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene. / Bhattacharya, Sanjoy K; Dubey, Ashok K.

In: Biotechnology Letters, Vol. 17, No. 11, 01.11.1995, p. 1155-1160.

Research output: Contribution to journalArticle

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