Metabolic and pathological effects of temporal lobe epilepsy in rat brain detected by proton spectroscopy and imaging

Takaaki Tokumitsu, Anthony Mancuso, Philip R. Weinstein, Michael W. Weiner, Shoji Naruse, Andrew A Maudsley

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The goal of these experiments was to test the hypothesis that in an animal model of temporal lobe epilepsy (TLE), magnetic resonance spectroscopic measurement of N-acetylaspartate (NAA) and other metabolites, together with magnetic resonance imaging, provides a sensitive in vivo method to localize and monitor the progression of neuronal cell death and gliosis. Seizures were induced in rats by unilateral hippocampal injection of kainate. Magnetic resonance measurements were made from 1 to 84 days using proton spectroscopic imaging (1H-MRSI), T2-weighted imaging (T2WI) and diffusion-weighted imaging (DWI). The results were compared with findings on histological sections. Decreased NAA and creatine levels and increased apparent diffusion coefficient of water were found in the ipsilateral hippocampus after 14 days where neuronal loss and gliosis were observed. In the contralateral hippocampus a significant increase of choline level was observed. These results suggest that 1H-MRSI is a useful in vivo method for localizing neuronal loss and may also indicate additional pathological and metabolic alterations. In addition, DWI may be a useful method for in vivo detection of tissue alterations due to TLE.

Original languageEnglish
Pages (from-to)57-67
Number of pages11
JournalBrain Research
Volume744
Issue number1
DOIs
StatePublished - Jan 2 1997
Externally publishedYes

Fingerprint

Temporal Lobe Epilepsy
Protons
Spectrum Analysis
Gliosis
Hippocampus
Brain
Magnetic Resonance Spectroscopy
Creatine
Kainic Acid
Choline
Seizures
Cell Death
Animal Models
Magnetic Resonance Imaging
Injections
Water
N-acetylaspartate

Keywords

  • choline
  • creatine
  • diffusion-weighted imaging
  • epilepsy
  • kainate
  • magnetic resonance spectroscopic imaging
  • N-acetylaspartate
  • rat brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Metabolic and pathological effects of temporal lobe epilepsy in rat brain detected by proton spectroscopy and imaging. / Tokumitsu, Takaaki; Mancuso, Anthony; Weinstein, Philip R.; Weiner, Michael W.; Naruse, Shoji; Maudsley, Andrew A.

In: Brain Research, Vol. 744, No. 1, 02.01.1997, p. 57-67.

Research output: Contribution to journalArticle

Tokumitsu, Takaaki ; Mancuso, Anthony ; Weinstein, Philip R. ; Weiner, Michael W. ; Naruse, Shoji ; Maudsley, Andrew A. / Metabolic and pathological effects of temporal lobe epilepsy in rat brain detected by proton spectroscopy and imaging. In: Brain Research. 1997 ; Vol. 744, No. 1. pp. 57-67.
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