Messenger RNA methylation, translation and degradation in extracts of interferon-treated cells.

G. C. Sen, Ronald Charles Desrosiers, L. Ratner, S. Shaila, G. E. Brown, B. Lebleu, E. Slattery, M. Kawakita, B. Cabrer, H. Taira, P. Lengyel

Research output: Contribution to journalArticle

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Abstract

Extracts from interferon-treated, not virus infected EAT cells differ in several biochemical characteristics from extracts of untreated cells. Some of these differences are manifested only if the extracts are supplemented with ds RNA and ATP. Thus, in the extracts from interferon-treated cells these supplements activate a protein kinase and an endonuclease activity as well as an inhibitor of the translation of messenger RNA. The effect of the same supplements in extracts of untreated cells is much less pronounced. Other differences between the two types of extracts do not seem to depend on the addition of ds RNA and ATP. These include an impairment of mRNA cap methylation and an inhibition of peptide chain elongation that can be overcome by the addition of tRNA. The treatment of human (HeLa S3) cells with human interferon is manifested in the cell extract similarly to the treatment of EAT cells with mouse interferon. Studies are underway to isolate and characterize the ds RNA activated enzymes and the inhibitors and to establish how the presence of these in extracts from interferon-treated cells can account for the impairment of virus replication by interferon.

Original languageEnglish (US)
Pages (from-to)221-229
Number of pages9
JournalTexas reports on biology and medicine
Volume35
StatePublished - 1977
Externally publishedYes

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Interferons
Methylation
Messenger RNA
Cell Extracts
RNA
Adenosine Triphosphate
Endonucleases
Enzyme Inhibitors
Virus Replication
Transfer RNA
HeLa Cells
Protein Kinases
Viruses
Peptides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sen, G. C., Desrosiers, R. C., Ratner, L., Shaila, S., Brown, G. E., Lebleu, B., ... Lengyel, P. (1977). Messenger RNA methylation, translation and degradation in extracts of interferon-treated cells. Texas reports on biology and medicine, 35, 221-229.

Messenger RNA methylation, translation and degradation in extracts of interferon-treated cells. / Sen, G. C.; Desrosiers, Ronald Charles; Ratner, L.; Shaila, S.; Brown, G. E.; Lebleu, B.; Slattery, E.; Kawakita, M.; Cabrer, B.; Taira, H.; Lengyel, P.

In: Texas reports on biology and medicine, Vol. 35, 1977, p. 221-229.

Research output: Contribution to journalArticle

Sen, GC, Desrosiers, RC, Ratner, L, Shaila, S, Brown, GE, Lebleu, B, Slattery, E, Kawakita, M, Cabrer, B, Taira, H & Lengyel, P 1977, 'Messenger RNA methylation, translation and degradation in extracts of interferon-treated cells.', Texas reports on biology and medicine, vol. 35, pp. 221-229.
Sen, G. C. ; Desrosiers, Ronald Charles ; Ratner, L. ; Shaila, S. ; Brown, G. E. ; Lebleu, B. ; Slattery, E. ; Kawakita, M. ; Cabrer, B. ; Taira, H. ; Lengyel, P. / Messenger RNA methylation, translation and degradation in extracts of interferon-treated cells. In: Texas reports on biology and medicine. 1977 ; Vol. 35. pp. 221-229.
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AU - Desrosiers, Ronald Charles

AU - Ratner, L.

AU - Shaila, S.

AU - Brown, G. E.

AU - Lebleu, B.

AU - Slattery, E.

AU - Kawakita, M.

AU - Cabrer, B.

AU - Taira, H.

AU - Lengyel, P.

PY - 1977

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