Abstract
Mesenchymal stem cells°MSC) may transdifferentiate into neural cells in vitro under the influence of matrix molecules and growth factors present in neurogenic niches. However, further experiments on the behavior of such stem cells remain to be done in vivo. In this study, rat MSC (rMSC) have been grafted in a neurogenic environment of the rat brain, the subventricular zone (SVZ), in order to detect and follow their migration using superparamagnetic iron oxide (SPIO) nanoparticles. We sought to characterize the potential effect of iron loading on the behavior of rMSC as well as to address the potential of rMSC to migrate when exposed to the adequate brain microenvironment. 1-hydroxyethylidene-1.1-bisphosphonic acid (HEDP)-coated SPIO nanoparticles efficiently labeled rMSC without significant adverse effects on cell viability and on the in vitro differentiation potential. In opposition to iron-labeled rat neural stem cells (rNSC), used as a positive control, iron-labeled rMSC did not respond to the SVZ microenvironment in vivo and did not migrate, unless a mechanical lesion of the olfactory bulb was performed. This confirmed the known potential of iron-labeled rMSC to migrate toward lesions and, as far as we know, this is the first study describing such a long distance migration from the SVZ toward the olfactory bulb through the rostral migratory stream (RMS).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 18-31 |
| Number of pages | 14 |
| Journal | Brain Research |
| Volume | 1255 |
| DOIs | |
| State | Published - Feb 19 2009 |
| Externally published | Yes |
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Keywords
- Cell migration
- Magnetic resonance imaging
- Mesenchymal stem cell
- Olfactory bulb
- SPIO
- Subventricular zone
ASJC Scopus subject areas
- Neuroscience(all)
- Clinical Neurology
- Developmental Biology
- Molecular Biology
Cite this
Mesenchymal and neural stem cells labeled with HEDP-coated SPIO nanoparticles : In vitro characterization and migration potential in rat brain. / Delcroix, Gaëtan J R; Jacquart, Matthieu; Lemaire, Laurent; Sindji, Laurence; Franconi, Florence; Le Jeune, Jean Jacques; Montero-Menei, Claudia N.
In: Brain Research, Vol. 1255, 19.02.2009, p. 18-31.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mesenchymal and neural stem cells labeled with HEDP-coated SPIO nanoparticles
T2 - In vitro characterization and migration potential in rat brain
AU - Delcroix, Gaëtan J R
AU - Jacquart, Matthieu
AU - Lemaire, Laurent
AU - Sindji, Laurence
AU - Franconi, Florence
AU - Le Jeune, Jean Jacques
AU - Montero-Menei, Claudia N.
PY - 2009/2/19
Y1 - 2009/2/19
N2 - Mesenchymal stem cells°MSC) may transdifferentiate into neural cells in vitro under the influence of matrix molecules and growth factors present in neurogenic niches. However, further experiments on the behavior of such stem cells remain to be done in vivo. In this study, rat MSC (rMSC) have been grafted in a neurogenic environment of the rat brain, the subventricular zone (SVZ), in order to detect and follow their migration using superparamagnetic iron oxide (SPIO) nanoparticles. We sought to characterize the potential effect of iron loading on the behavior of rMSC as well as to address the potential of rMSC to migrate when exposed to the adequate brain microenvironment. 1-hydroxyethylidene-1.1-bisphosphonic acid (HEDP)-coated SPIO nanoparticles efficiently labeled rMSC without significant adverse effects on cell viability and on the in vitro differentiation potential. In opposition to iron-labeled rat neural stem cells (rNSC), used as a positive control, iron-labeled rMSC did not respond to the SVZ microenvironment in vivo and did not migrate, unless a mechanical lesion of the olfactory bulb was performed. This confirmed the known potential of iron-labeled rMSC to migrate toward lesions and, as far as we know, this is the first study describing such a long distance migration from the SVZ toward the olfactory bulb through the rostral migratory stream (RMS).
AB - Mesenchymal stem cells°MSC) may transdifferentiate into neural cells in vitro under the influence of matrix molecules and growth factors present in neurogenic niches. However, further experiments on the behavior of such stem cells remain to be done in vivo. In this study, rat MSC (rMSC) have been grafted in a neurogenic environment of the rat brain, the subventricular zone (SVZ), in order to detect and follow their migration using superparamagnetic iron oxide (SPIO) nanoparticles. We sought to characterize the potential effect of iron loading on the behavior of rMSC as well as to address the potential of rMSC to migrate when exposed to the adequate brain microenvironment. 1-hydroxyethylidene-1.1-bisphosphonic acid (HEDP)-coated SPIO nanoparticles efficiently labeled rMSC without significant adverse effects on cell viability and on the in vitro differentiation potential. In opposition to iron-labeled rat neural stem cells (rNSC), used as a positive control, iron-labeled rMSC did not respond to the SVZ microenvironment in vivo and did not migrate, unless a mechanical lesion of the olfactory bulb was performed. This confirmed the known potential of iron-labeled rMSC to migrate toward lesions and, as far as we know, this is the first study describing such a long distance migration from the SVZ toward the olfactory bulb through the rostral migratory stream (RMS).
KW - Cell migration
KW - Magnetic resonance imaging
KW - Mesenchymal stem cell
KW - Olfactory bulb
KW - SPIO
KW - Subventricular zone
UR - http://www.scopus.com/inward/record.url?scp=59249089568&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59249089568&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2008.12.013
DO - 10.1016/j.brainres.2008.12.013
M3 - Article
C2 - 19103182
AN - SCOPUS:59249089568
VL - 1255
SP - 18
EP - 31
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -