Membrane‐associated proteins of adriamycin sensitive and resistant murine leukemic P388 cells

NABILA M. SHANBAKY, T. S.ANANTHA SAMY, ROBERT RUBIN, AWTAR KRISHAN

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We have isolated an 84‐fold adriamycin resistant subline, P388/R84, from mouse leukemia P388 cells by serial cultivation in methylcellulose in the presence of increasing drug concentrations. Electrophoresis of detergent soluble fractions of radiolabeled sensitive and resistant cells suggested marked alterations in the protein fractions of 160, 100, 60, 45, and 30 kd. In resistant clones labeled with 1251 an increase in 160 and 100 kd proteins was accompanied by concomitant reduction in the 60, 45, and 30 kd proteins. In 35S methionine‐labeled resistant cells, similar increases in the 160 and 100 kd components were observed but in contrast to 125I‐labeled cells the 30 kd component was also higher. Alterations in surface proteins were confirmed in experiments where the cell extracts were adsorbed to concanavalin A polymers and extracted with 0.26 M methyl‐α‐D‐mannopyranoside. Our data confirm earlier reported observations on cell‐surface protein changes in cells resistant to anthracyclines and alkaloids.

Original languageEnglish (US)
Pages (from-to)414-420
Number of pages7
JournalInternational journal of peptide and protein research
Volume27
Issue number4
DOIs
StatePublished - Apr 1986

Keywords

  • 160 kd‐glycoprotein
  • adriamycin
  • membrane proteins
  • resistant murine leukemic cells
  • sensitive
  • two‐dimensional electrophoresis

ASJC Scopus subject areas

  • Biochemistry

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