Membrane phenotype and expression of perforin and serine esterases by CD3- peripheral blood and decidual granular lymphocyte-derived clones

L. Gudelj, S. E. Christmas, G. Laskarin, P. M. Johnson, E. R. Podack, D. Rukavina

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Problem: Human first-trimester pregnancy decidua were found to contain large numbers of perforin (P)-containing cells, which varied in their membrane antigen phenotype. In this study results obtained by analyzing CD3- clones derived from human early pregnancy decidua and peripheral blood are reported. Method of Study: Decidual tissue was obtained from vaginal termination of first trimester normal human pregnancies. CD3- clones were generated by limiting dilution cloning after the depletion of CD3+ lymphocytes. The cell membrane phenotype was determined by flow cytometry. Perforin was detected by fluorescence-activated cell sorter (FACS) analysis of permeabilised cells. Serine esterases (SE) were identified by histochemical staining for BLT-esterase. Results: Cloned decidual cell populations retained the overall antigenic phenotype of freshly isolated decidual natural killer (NK)-like cells. All CD3- clones, either derived from decidua or from peripheral blood contained perforin. Serine esterases were present in every decidual clone analyzed. Conclusions: Limiting dilution cloning allows the clear-cut analysis of homogenous subsets of decidua-derived NK-like clones. The presence of large amounts of perforin in all of the CD3- clones underlines the extensive transcription of the perforin gene by NK-like lymphocytes.

Original languageEnglish (US)
Pages (from-to)162-167
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume38
Issue number3
DOIs
StatePublished - 1997

Keywords

  • CD3 clones
  • Decidual granular lymphocytes
  • Perforin
  • Peripheral blood lymphocytes
  • Serine esterases

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

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