Membrane localization and topology of the Yersinia pestis YscJ lipoprotein

Eugenia Silva-Herzog, Franco Ferracci, Michael W. Jackson, Sabrina S. Joseph, Gregory V Plano

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The localization and membrane topology of the Yersinia pestis. YscJ lipoprotein an essential component of the type III secretion apparatus, was investigated: YscJ was demonstrated to be an inner membrane (IM) lipoprotein that is anchored to the periplasmic face of the IM via an N-terminal lipid moiety and via a C-terminal transmembrane (TM) domain. Localization of the N-terminal lipid moiety to the IM occurred regardless of the amino-acid residues found in the +2 or +3 positions. IM localization was dependent upon an intact N-terminal domain (amino acids + 1 to + 61), suggesting that this region plays a role in YscJ localization. In contrast, the YscJ C-terminal domain and TM domain were not required for IM localization N-terminal sequence analysis demonstrated that a significant proportion of membrane-localized YscJ laoks N-acylation, the final modification required for Lol-dependent transport of a lipoprotein to the OM. Interestingly, attachment of the N-terminus to the IM was required for YscJ function; however, the YscJ secretion signal and lipo-box could be functionally replaced by the first TM domain of the YscV protein, suggesting that the mechanism of attachment to the IM was not critical.

Original languageEnglish
Pages (from-to)593-607
Number of pages15
JournalMicrobiology
Volume154
Issue number2
DOIs
StatePublished - Feb 1 2008

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Yersinia pestis
Lipoproteins
Membranes
Lipids
Amino Acids
Acylation
Sequence Analysis

ASJC Scopus subject areas

  • Microbiology

Cite this

Membrane localization and topology of the Yersinia pestis YscJ lipoprotein. / Silva-Herzog, Eugenia; Ferracci, Franco; Jackson, Michael W.; Joseph, Sabrina S.; Plano, Gregory V.

In: Microbiology, Vol. 154, No. 2, 01.02.2008, p. 593-607.

Research output: Contribution to journalArticle

Silva-Herzog, E, Ferracci, F, Jackson, MW, Joseph, SS & Plano, GV 2008, 'Membrane localization and topology of the Yersinia pestis YscJ lipoprotein', Microbiology, vol. 154, no. 2, pp. 593-607. https://doi.org/10.1099/mic.0.2007/013045-0
Silva-Herzog, Eugenia ; Ferracci, Franco ; Jackson, Michael W. ; Joseph, Sabrina S. ; Plano, Gregory V. / Membrane localization and topology of the Yersinia pestis YscJ lipoprotein. In: Microbiology. 2008 ; Vol. 154, No. 2. pp. 593-607.
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