Megakaryocyte development is normal in mice with targeted disruption of Tescalcin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H + exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and human CD34 + hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation. Objective: To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis. Methods: We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development. Results: Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal. Conclusion: Tescalcin does not appear to be necessary for normal megakaryocyte development.

Original languageEnglish
Pages (from-to)662-669
Number of pages8
JournalExperimental Cell Research
Volume318
Issue number5
DOIs
StatePublished - Mar 10 2012

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Megakaryocytes
Sodium-Hydrogen Antiporter
EF Hand Motifs
Calcium-Binding Proteins
K562 Cells
Hematopoietic Stem Cells
Platelet Count
Bone Marrow
Cell Line

Keywords

  • Fli-1
  • Megakaryocyte
  • NHE1
  • Platelet
  • Tescalcin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Megakaryocyte development is normal in mice with targeted disruption of Tescalcin. / Ukarapong, Supamit; Bao, Yong; Perera, Erasmo M.; Berkovitz, Gary.

In: Experimental Cell Research, Vol. 318, No. 5, 10.03.2012, p. 662-669.

Research output: Contribution to journalArticle

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abstract = "Background: Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H + exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and human CD34 + hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation. Objective: To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis. Methods: We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development. Results: Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal. Conclusion: Tescalcin does not appear to be necessary for normal megakaryocyte development.",
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AU - Perera, Erasmo M.

AU - Berkovitz, Gary

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N2 - Background: Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H + exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and human CD34 + hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation. Objective: To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis. Methods: We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development. Results: Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal. Conclusion: Tescalcin does not appear to be necessary for normal megakaryocyte development.

AB - Background: Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H + exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and human CD34 + hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation. Objective: To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis. Methods: We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development. Results: Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal. Conclusion: Tescalcin does not appear to be necessary for normal megakaryocyte development.

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