MEF, a novel transcription factor with an Elf-1 like DNA binding domain but distinct transcriptional activating properties

Yasushi Miyazaki, Xiao Sun, Hideo Uchida, Jin Zhang, Stephen D Nimer

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

To isolate novel ETS genes expressed in hematopoietic cells we used a nested, degenerate oligonucleotide PCR based technique to obtain a cDNA fragment that encodes an ETS domain from a human megakaryocytic cell line. This fragment was used to isolate a full length, 4.2 kb cDNA that encodes a 663 amino acid protein which we call MEF (myeloid elf-1-like factor). MEF contains a central ETS domain and is expressed in a variety of myeloid leukemia cell lines and in normal adult hematopoietic tissue such as spleen, thymus and peripheral blood, as well as non-hematopoietic tissue such as ovary, placenta and colon. Using an oligonucleotide binding selection assay, and bacterially expressed MEF, we demonstrated that MEF recognizes a consensus DNA binding sequence nearly identical to elf-1 (..WGGA..) and then showed that MEF specifically bound to known elf-1 binding sites in the human GMCSF and IL-3 promoters. To characterize the functional effect of MEF on transcription, we performed co-transfection experiments in myeloid cells, and T cells, and demonstrated transactivation of both promoters by MEF, but not by elf-1. MEF is a new member of the elf-1/E74 family of ETS proteins, that has DNA binding properties similar to elf-1 but distinct transcriptional activating effects in several hematopoietic cell lineages.

Original languageEnglish
Pages (from-to)1721-1729
Number of pages9
JournalOncogene
Volume13
Issue number8
StatePublished - Nov 20 1996
Externally publishedYes

Fingerprint

Myeloid Cells
Oligonucleotides
Transcription Factors
Complementary DNA
Cell Line
Myeloid Leukemia
Interleukin-3
DNA
DNA-Binding Proteins
Cell Lineage
Thymus Gland
Placenta
Transcriptional Activation
Transfection
Ovary
Colon
Spleen
Binding Sites
T-Lymphocytes
Amino Acids

Keywords

  • Cytokine genes
  • ETS protein
  • Transcriptional activator

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

MEF, a novel transcription factor with an Elf-1 like DNA binding domain but distinct transcriptional activating properties. / Miyazaki, Yasushi; Sun, Xiao; Uchida, Hideo; Zhang, Jin; Nimer, Stephen D.

In: Oncogene, Vol. 13, No. 8, 20.11.1996, p. 1721-1729.

Research output: Contribution to journalArticle

Miyazaki, Yasushi ; Sun, Xiao ; Uchida, Hideo ; Zhang, Jin ; Nimer, Stephen D. / MEF, a novel transcription factor with an Elf-1 like DNA binding domain but distinct transcriptional activating properties. In: Oncogene. 1996 ; Vol. 13, No. 8. pp. 1721-1729.
@article{5c0dedfeab9d4d39b77dbb110b493047,
title = "MEF, a novel transcription factor with an Elf-1 like DNA binding domain but distinct transcriptional activating properties",
abstract = "To isolate novel ETS genes expressed in hematopoietic cells we used a nested, degenerate oligonucleotide PCR based technique to obtain a cDNA fragment that encodes an ETS domain from a human megakaryocytic cell line. This fragment was used to isolate a full length, 4.2 kb cDNA that encodes a 663 amino acid protein which we call MEF (myeloid elf-1-like factor). MEF contains a central ETS domain and is expressed in a variety of myeloid leukemia cell lines and in normal adult hematopoietic tissue such as spleen, thymus and peripheral blood, as well as non-hematopoietic tissue such as ovary, placenta and colon. Using an oligonucleotide binding selection assay, and bacterially expressed MEF, we demonstrated that MEF recognizes a consensus DNA binding sequence nearly identical to elf-1 (..WGGA..) and then showed that MEF specifically bound to known elf-1 binding sites in the human GMCSF and IL-3 promoters. To characterize the functional effect of MEF on transcription, we performed co-transfection experiments in myeloid cells, and T cells, and demonstrated transactivation of both promoters by MEF, but not by elf-1. MEF is a new member of the elf-1/E74 family of ETS proteins, that has DNA binding properties similar to elf-1 but distinct transcriptional activating effects in several hematopoietic cell lineages.",
keywords = "Cytokine genes, ETS protein, Transcriptional activator",
author = "Yasushi Miyazaki and Xiao Sun and Hideo Uchida and Jin Zhang and Nimer, {Stephen D}",
year = "1996",
month = "11",
day = "20",
language = "English",
volume = "13",
pages = "1721--1729",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - MEF, a novel transcription factor with an Elf-1 like DNA binding domain but distinct transcriptional activating properties

AU - Miyazaki, Yasushi

AU - Sun, Xiao

AU - Uchida, Hideo

AU - Zhang, Jin

AU - Nimer, Stephen D

PY - 1996/11/20

Y1 - 1996/11/20

N2 - To isolate novel ETS genes expressed in hematopoietic cells we used a nested, degenerate oligonucleotide PCR based technique to obtain a cDNA fragment that encodes an ETS domain from a human megakaryocytic cell line. This fragment was used to isolate a full length, 4.2 kb cDNA that encodes a 663 amino acid protein which we call MEF (myeloid elf-1-like factor). MEF contains a central ETS domain and is expressed in a variety of myeloid leukemia cell lines and in normal adult hematopoietic tissue such as spleen, thymus and peripheral blood, as well as non-hematopoietic tissue such as ovary, placenta and colon. Using an oligonucleotide binding selection assay, and bacterially expressed MEF, we demonstrated that MEF recognizes a consensus DNA binding sequence nearly identical to elf-1 (..WGGA..) and then showed that MEF specifically bound to known elf-1 binding sites in the human GMCSF and IL-3 promoters. To characterize the functional effect of MEF on transcription, we performed co-transfection experiments in myeloid cells, and T cells, and demonstrated transactivation of both promoters by MEF, but not by elf-1. MEF is a new member of the elf-1/E74 family of ETS proteins, that has DNA binding properties similar to elf-1 but distinct transcriptional activating effects in several hematopoietic cell lineages.

AB - To isolate novel ETS genes expressed in hematopoietic cells we used a nested, degenerate oligonucleotide PCR based technique to obtain a cDNA fragment that encodes an ETS domain from a human megakaryocytic cell line. This fragment was used to isolate a full length, 4.2 kb cDNA that encodes a 663 amino acid protein which we call MEF (myeloid elf-1-like factor). MEF contains a central ETS domain and is expressed in a variety of myeloid leukemia cell lines and in normal adult hematopoietic tissue such as spleen, thymus and peripheral blood, as well as non-hematopoietic tissue such as ovary, placenta and colon. Using an oligonucleotide binding selection assay, and bacterially expressed MEF, we demonstrated that MEF recognizes a consensus DNA binding sequence nearly identical to elf-1 (..WGGA..) and then showed that MEF specifically bound to known elf-1 binding sites in the human GMCSF and IL-3 promoters. To characterize the functional effect of MEF on transcription, we performed co-transfection experiments in myeloid cells, and T cells, and demonstrated transactivation of both promoters by MEF, but not by elf-1. MEF is a new member of the elf-1/E74 family of ETS proteins, that has DNA binding properties similar to elf-1 but distinct transcriptional activating effects in several hematopoietic cell lineages.

KW - Cytokine genes

KW - ETS protein

KW - Transcriptional activator

UR - http://www.scopus.com/inward/record.url?scp=0029860814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029860814&partnerID=8YFLogxK

M3 - Article

C2 - 8895518

AN - SCOPUS:0029860814

VL - 13

SP - 1721

EP - 1729

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 8

ER -