Mediation of oviposition site selection in the African malaria mosquito Anopheles gambiae (Diptera: Culicidae) by semiochemicals of microbial origin

Leunita A. Sumba, Tom O. Guda, Arop L. Deng, Ahmed Hassanali, John C Beier, Bart G J Knols

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Laboratory studies were carried out to investigate the role of larval habitat-derived microorganisms in the production of semiochemicals for oviposition site selection by Anopheles gambiae Giles sensu stricto mosquitoes. Dual-choice bioassays with gravid females were conducted in standard mosquito cages. Field-collected or laboratory-reared mosquitoes, individually or in groups, were offered a choice between unmodified (water or soil from a natural breeding site) or modified substrates (filtered water, autoclaved soil or sterile media to which bacterial suspensions had been added). Egg counts were used to assess oviposition preferences. Mosquitoes preferred to oviposit on unmodified substrates from natural larval habitats containing live microorganisms rather than on sterilized ones. Variable responses were observed when sterile substrates were inoculated with bacteria isolated from water and soil from natural habitats. We conclude that microbial populations in breeding sites produce volatiles that serve as semiochemicals for gravid An. gambiae. These signals, in conjunction with other (non-olfactory) chemical and physical cues, may be used by the female to assess the suitability of potential larval habitats in order to maximize the fitness of her offspring.

Original languageEnglish
Pages (from-to)260-265
Number of pages6
JournalInternational Journal of Tropical Insect Science
Volume24
Issue number3
DOIs
StatePublished - Sep 1 2004

Fingerprint

semiochemical
semiochemicals
oviposition sites
Anopheles gambiae
malaria
site selection
mosquito
oviposition
Culicidae
breeding site
habitat
habitats
substrate
breeding sites
microorganism
microorganisms
gravid females
bioassay
soil
cages

Keywords

  • Anopheles gambiae
  • Oviposition site selection
  • Semiochemicals
  • Soil microbiota

ASJC Scopus subject areas

  • Insect Science

Cite this

Mediation of oviposition site selection in the African malaria mosquito Anopheles gambiae (Diptera : Culicidae) by semiochemicals of microbial origin. / Sumba, Leunita A.; Guda, Tom O.; Deng, Arop L.; Hassanali, Ahmed; Beier, John C; Knols, Bart G J.

In: International Journal of Tropical Insect Science, Vol. 24, No. 3, 01.09.2004, p. 260-265.

Research output: Contribution to journalArticle

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