Mechanosensitive release of adenosine 5′-triphosphate through pannexin channels and mechanosensitive upregulation of pannexin channels in optic nerve head astrocytes: A mechanism for purinergic involvement in chronic strain

Jonathan M. Beckel, Arthur J. Argall, Jason C. Lim, Jingsheng Xia, Wennan Lu, Erin E. Coffey, Edward J. Macarak, Mohammed Shahidullah, Nicholas A. Delamere, Gulab S. Zode, Val C. Sheffield, Valery I Shestopalov, Alan M. Laties, Claire H. Mitchell

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

As adenosine 5′-triphosphate (ATP) released from astrocytes can modulate many neural signaling systems, the triggers and pathways for this ATP release are important. Here, the ability of mechanical strain to trigger ATP release through pannexin channels and the effects of sustained strain on pannexin expression were examined in rat optic nerve head astrocytes. Astrocytes released ATP when subjected to 5% of equibiaxial strain or to hypotonic swelling. Although astrocytes expressed mRNA for pannexins 1-3, connexin 43, and VNUT, pharmacological analysis suggested a predominant role for pannexins in mechanosensitive ATP release, with Rho kinase contribution. Astrocytes from panx1-/- mice had reduced baseline and stimulated levels of extracellular ATP, confirming the role for pannexins. Swelling astrocytes triggered a regulatory volume decrease that was inhibited by apyrase or probenecid. The swelling-induced rise in calcium was inhibited by P2X7 receptor antagonists A438079 and AZ10606120, in addition to apyrase and carbenoxolone. Extended stretch of astrocytes in vitro upregulated expression of panx1 and panx2 mRNA. A similar upregulation was observed in vivo in optic nerve head tissue from the Tg-MYOCY437H mouse model of chronic glaucoma; genes for panx1, panx2, and panx3 were increased, whereas immunohistochemistry confirmed increased expression of pannexin 1 protein. In summary, astrocytes released ATP in response to mechanical strain, with pannexin 1 the predominant efflux pathway. Sustained strain upregulated pannexins in vitro and in vivo. Together, these findings provide a mechanism by which extracellular ATP remains elevated under chronic mechanical strain, as found in the optic nerve head of patients with glaucoma.

Original languageEnglish
Pages (from-to)1486-1501
Number of pages16
JournalGLIA
Volume62
Issue number9
DOIs
StatePublished - Jan 1 2014

Fingerprint

Optic Disk
Astrocytes
Up-Regulation
Adenosine Triphosphate
Apyrase
Glaucoma
Carbenoxolone
Purinergic P2X7 Receptors
Probenecid
Nerve Tissue
rho-Associated Kinases
Messenger RNA
Connexin 43
Immunohistochemistry
Pharmacology
Calcium
Genes

Keywords

  • ATP release
  • Cell swelling
  • Glaucoma
  • Mechanosensitive signaling
  • P2X7 receptor
  • Pannexin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Mechanosensitive release of adenosine 5′-triphosphate through pannexin channels and mechanosensitive upregulation of pannexin channels in optic nerve head astrocytes : A mechanism for purinergic involvement in chronic strain. / Beckel, Jonathan M.; Argall, Arthur J.; Lim, Jason C.; Xia, Jingsheng; Lu, Wennan; Coffey, Erin E.; Macarak, Edward J.; Shahidullah, Mohammed; Delamere, Nicholas A.; Zode, Gulab S.; Sheffield, Val C.; Shestopalov, Valery I; Laties, Alan M.; Mitchell, Claire H.

In: GLIA, Vol. 62, No. 9, 01.01.2014, p. 1486-1501.

Research output: Contribution to journalArticle

Beckel, JM, Argall, AJ, Lim, JC, Xia, J, Lu, W, Coffey, EE, Macarak, EJ, Shahidullah, M, Delamere, NA, Zode, GS, Sheffield, VC, Shestopalov, VI, Laties, AM & Mitchell, CH 2014, 'Mechanosensitive release of adenosine 5′-triphosphate through pannexin channels and mechanosensitive upregulation of pannexin channels in optic nerve head astrocytes: A mechanism for purinergic involvement in chronic strain', GLIA, vol. 62, no. 9, pp. 1486-1501. https://doi.org/10.1002/glia.22695
Beckel, Jonathan M. ; Argall, Arthur J. ; Lim, Jason C. ; Xia, Jingsheng ; Lu, Wennan ; Coffey, Erin E. ; Macarak, Edward J. ; Shahidullah, Mohammed ; Delamere, Nicholas A. ; Zode, Gulab S. ; Sheffield, Val C. ; Shestopalov, Valery I ; Laties, Alan M. ; Mitchell, Claire H. / Mechanosensitive release of adenosine 5′-triphosphate through pannexin channels and mechanosensitive upregulation of pannexin channels in optic nerve head astrocytes : A mechanism for purinergic involvement in chronic strain. In: GLIA. 2014 ; Vol. 62, No. 9. pp. 1486-1501.
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AU - Lim, Jason C.

AU - Xia, Jingsheng

AU - Lu, Wennan

AU - Coffey, Erin E.

AU - Macarak, Edward J.

AU - Shahidullah, Mohammed

AU - Delamere, Nicholas A.

AU - Zode, Gulab S.

AU - Sheffield, Val C.

AU - Shestopalov, Valery I

AU - Laties, Alan M.

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