Mechanisms Underlying the Dual Effect of Polyunsaturated Fatty Acid Analogs on Kv7.1

Sara I. Liin, Samira Yazdi, Rosamary Ramentol, Rene Barro, Hans P Larsson

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Polyunsaturated fatty acid (PUFA) analogs represent a new class of potential anti-arrhythmic KV7.1 and KV7.1+KCNE1 channel activators. In this study, we describe dual independent activating effects of negatively charged PUFA analogs on KV7.1 and KV7.1+KCNE1 that are dependent on discrete channel motifs. PUFA analogs are critically dependent on K326 in S6 of KV7.1 to increase the maximum conductance and critically dependent on specific S4 arginines in KV7.1 to shift the voltage dependence of channel opening toward negative voltages. Our findings provide insights into how KV7.1+KCNE1 activators may interact electrostatically both with the pore domain and the voltage-sensing domain to augment channel activity. We believe that the molecular understanding of how PUFA analogs induce dual independent activating effects is an important step toward the development of effective anti-arrhythmic drugs that target KV7.1 channels. Polyunsaturated fatty acid (PUFA) analogs are potentially anti-arrhythmic compounds, and understanding their functional mechanisms can aid in rational drug design. Liin et al. find that negatively charged PUFA analogs activate the cardiac potassium channel KV7.1 by dual independent mechanisms, demonstrating augmentation of channel activity through electrostatic interactions with both the pore and voltage-sensing domains of KV7.1.

Original languageEnglish (US)
Pages (from-to)2908-2918
Number of pages11
JournalCell Reports
Volume24
Issue number11
DOIs
StatePublished - Sep 11 2018

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Keywords

  • cardiac arrhythmia
  • I activator
  • I channel
  • KCNE1
  • KCNQ1
  • polyunsaturated fatty acid
  • two-electrode voltage clamp
  • Xenopus oocyte

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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