Abstract
T cells that express the γδ T-cell receptor, which recognize microbial or stress-induced antigens, represent a minority of blood T cells but constitute a major proportion of intraepithelial lymphocytes in the gastrointestinal mucosa. As microbial products have been shown to translocate from the gastrointestinal tract into circulation in chronically HIV/Simian immunodeficiency virus (SIV) - infected individuals, we conducted a study of Vδ1 and Vδ2 T-cell frequency, phenotype, and function in blood, spleen, lymph nodes, gastrointestinal mucosa, and bronchoalveolar lavage of uninfected and chronically SIVsmE543-infected rhesus macaques (RMs).We found: (1) SIV-associated inversion of Vδ1/Vδ2 T cells occurs in blood and in several tissues; (2) γδ T cells are not infected by SIV in vivo; (3) the Vδ1/ Vδ2 inversion involves expansion of Vδ1 T cells; (4) expanded Vδ1 T cells are phenotypically and functionally different from Vδ1 T cells from uninfected RMs; and (5) the stimulus underlying expansion of Vδ1 T cells appears to be microbial translocation. These data highlight the importance of microbial translocation - induced immune activation in chronically infected individuals and provide new insights into an immune dysregulation phenomenon that is a hallmark of HIV/SIV infection. These findings may lead to novel therapeutic interventions that improve the immune responses against microbial antigens, and thus, decrease microbial translocation - induced immune activation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4148-4157 |
| Number of pages | 10 |
| Journal | Blood |
| Volume | 116 |
| Issue number | 20 |
| DOIs | |
| State | Published - Nov 18 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Biochemistry
- Hematology
- Cell Biology
Cite this
Mechanisms underlying γδ T-cell subset perturbations in SIV-infected Asian rhesus macaques. / Harris, Levelle D.; Klatt, Nichole; Vinton, Carol; Briant, Judith A.; Tabb, Brian; Ladell, Kristin; Lifson, Jeffrey; Estes, Jacob D.; Price, David A.; Hirsch, Vanessa M.; Brenchley, Jason M.
In: Blood, Vol. 116, No. 20, 18.11.2010, p. 4148-4157.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanisms underlying γδ T-cell subset perturbations in SIV-infected Asian rhesus macaques
AU - Harris, Levelle D.
AU - Klatt, Nichole
AU - Vinton, Carol
AU - Briant, Judith A.
AU - Tabb, Brian
AU - Ladell, Kristin
AU - Lifson, Jeffrey
AU - Estes, Jacob D.
AU - Price, David A.
AU - Hirsch, Vanessa M.
AU - Brenchley, Jason M.
PY - 2010/11/18
Y1 - 2010/11/18
N2 - T cells that express the γδ T-cell receptor, which recognize microbial or stress-induced antigens, represent a minority of blood T cells but constitute a major proportion of intraepithelial lymphocytes in the gastrointestinal mucosa. As microbial products have been shown to translocate from the gastrointestinal tract into circulation in chronically HIV/Simian immunodeficiency virus (SIV) - infected individuals, we conducted a study of Vδ1 and Vδ2 T-cell frequency, phenotype, and function in blood, spleen, lymph nodes, gastrointestinal mucosa, and bronchoalveolar lavage of uninfected and chronically SIVsmE543-infected rhesus macaques (RMs).We found: (1) SIV-associated inversion of Vδ1/Vδ2 T cells occurs in blood and in several tissues; (2) γδ T cells are not infected by SIV in vivo; (3) the Vδ1/ Vδ2 inversion involves expansion of Vδ1 T cells; (4) expanded Vδ1 T cells are phenotypically and functionally different from Vδ1 T cells from uninfected RMs; and (5) the stimulus underlying expansion of Vδ1 T cells appears to be microbial translocation. These data highlight the importance of microbial translocation - induced immune activation in chronically infected individuals and provide new insights into an immune dysregulation phenomenon that is a hallmark of HIV/SIV infection. These findings may lead to novel therapeutic interventions that improve the immune responses against microbial antigens, and thus, decrease microbial translocation - induced immune activation.
AB - T cells that express the γδ T-cell receptor, which recognize microbial or stress-induced antigens, represent a minority of blood T cells but constitute a major proportion of intraepithelial lymphocytes in the gastrointestinal mucosa. As microbial products have been shown to translocate from the gastrointestinal tract into circulation in chronically HIV/Simian immunodeficiency virus (SIV) - infected individuals, we conducted a study of Vδ1 and Vδ2 T-cell frequency, phenotype, and function in blood, spleen, lymph nodes, gastrointestinal mucosa, and bronchoalveolar lavage of uninfected and chronically SIVsmE543-infected rhesus macaques (RMs).We found: (1) SIV-associated inversion of Vδ1/Vδ2 T cells occurs in blood and in several tissues; (2) γδ T cells are not infected by SIV in vivo; (3) the Vδ1/ Vδ2 inversion involves expansion of Vδ1 T cells; (4) expanded Vδ1 T cells are phenotypically and functionally different from Vδ1 T cells from uninfected RMs; and (5) the stimulus underlying expansion of Vδ1 T cells appears to be microbial translocation. These data highlight the importance of microbial translocation - induced immune activation in chronically infected individuals and provide new insights into an immune dysregulation phenomenon that is a hallmark of HIV/SIV infection. These findings may lead to novel therapeutic interventions that improve the immune responses against microbial antigens, and thus, decrease microbial translocation - induced immune activation.
UR - http://www.scopus.com/inward/record.url?scp=78549236553&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78549236553&partnerID=8YFLogxK
U2 - 10.1182/blood-2010-05-283549
DO - 10.1182/blood-2010-05-283549
M3 - Article
C2 - 20660793
AN - SCOPUS:78549236553
VL - 116
SP - 4148
EP - 4157
JO - Blood
JF - Blood
SN - 0006-4971
IS - 20
ER -