Mechanisms underlying γδ T-cell subset perturbations in SIV-infected Asian rhesus macaques

Levelle D. Harris, Nichole Klatt, Carol Vinton, Judith A. Briant, Brian Tabb, Kristin Ladell, Jeffrey Lifson, Jacob D. Estes, David A. Price, Vanessa M. Hirsch, Jason M. Brenchley

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

T cells that express the γδ T-cell receptor, which recognize microbial or stress-induced antigens, represent a minority of blood T cells but constitute a major proportion of intraepithelial lymphocytes in the gastrointestinal mucosa. As microbial products have been shown to translocate from the gastrointestinal tract into circulation in chronically HIV/Simian immunodeficiency virus (SIV) - infected individuals, we conducted a study of Vδ1 and Vδ2 T-cell frequency, phenotype, and function in blood, spleen, lymph nodes, gastrointestinal mucosa, and bronchoalveolar lavage of uninfected and chronically SIVsmE543-infected rhesus macaques (RMs).We found: (1) SIV-associated inversion of Vδ1/Vδ2 T cells occurs in blood and in several tissues; (2) γδ T cells are not infected by SIV in vivo; (3) the Vδ1/ Vδ2 inversion involves expansion of Vδ1 T cells; (4) expanded Vδ1 T cells are phenotypically and functionally different from Vδ1 T cells from uninfected RMs; and (5) the stimulus underlying expansion of Vδ1 T cells appears to be microbial translocation. These data highlight the importance of microbial translocation - induced immune activation in chronically infected individuals and provide new insights into an immune dysregulation phenomenon that is a hallmark of HIV/SIV infection. These findings may lead to novel therapeutic interventions that improve the immune responses against microbial antigens, and thus, decrease microbial translocation - induced immune activation.

Original languageEnglish (US)
Pages (from-to)4148-4157
Number of pages10
JournalBlood
Volume116
Issue number20
DOIs
StatePublished - Nov 18 2010
Externally publishedYes

Fingerprint

Simian Immunodeficiency Virus
T-cells
T-Lymphocyte Subsets
Macaca mulatta
Viruses
T-Lymphocytes
Blood
Mucous Membrane
Chemical activation
HIV
Antigens
Lymphocytes
Bronchoalveolar Lavage
Virus Diseases
T-Cell Antigen Receptor
Gastrointestinal Tract
Blood Cells
Spleen
Lymph Nodes
Tissue

ASJC Scopus subject areas

  • Immunology
  • Biochemistry
  • Hematology
  • Cell Biology

Cite this

Harris, L. D., Klatt, N., Vinton, C., Briant, J. A., Tabb, B., Ladell, K., ... Brenchley, J. M. (2010). Mechanisms underlying γδ T-cell subset perturbations in SIV-infected Asian rhesus macaques. Blood, 116(20), 4148-4157. https://doi.org/10.1182/blood-2010-05-283549

Mechanisms underlying γδ T-cell subset perturbations in SIV-infected Asian rhesus macaques. / Harris, Levelle D.; Klatt, Nichole; Vinton, Carol; Briant, Judith A.; Tabb, Brian; Ladell, Kristin; Lifson, Jeffrey; Estes, Jacob D.; Price, David A.; Hirsch, Vanessa M.; Brenchley, Jason M.

In: Blood, Vol. 116, No. 20, 18.11.2010, p. 4148-4157.

Research output: Contribution to journalArticle

Harris, LD, Klatt, N, Vinton, C, Briant, JA, Tabb, B, Ladell, K, Lifson, J, Estes, JD, Price, DA, Hirsch, VM & Brenchley, JM 2010, 'Mechanisms underlying γδ T-cell subset perturbations in SIV-infected Asian rhesus macaques', Blood, vol. 116, no. 20, pp. 4148-4157. https://doi.org/10.1182/blood-2010-05-283549
Harris, Levelle D. ; Klatt, Nichole ; Vinton, Carol ; Briant, Judith A. ; Tabb, Brian ; Ladell, Kristin ; Lifson, Jeffrey ; Estes, Jacob D. ; Price, David A. ; Hirsch, Vanessa M. ; Brenchley, Jason M. / Mechanisms underlying γδ T-cell subset perturbations in SIV-infected Asian rhesus macaques. In: Blood. 2010 ; Vol. 116, No. 20. pp. 4148-4157.
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