Mechanisms of oxygen free radical-induced calcium overload in endothelial cells

Dido Franceschi, D. Graham, M. Sarasua, R. M. Zollinger

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Unregulated increases in intracellular calcium levels with subsequent cytoskeletal disruption have been implicated in tissue injury caused by oxygen free radicals (OFRs). The purpose of these experiments was to study the kinetics of free intracellular calcium in endothelial cells on exposure to exogenous OFRs and investigate the mechanisms of this altered homeostasis. Monolayers of endothelial cells were labeled with a calcium-sensitive probe and exposed to exogenous OFRs generated with hypoxanthine and xanthine oxidase. Intracellular calcium changes were monitored dynamically by continuous measurement of fluorescence with a spectrofluorometer. A sustained rise in intracellular Ca++ levels reaching a peak at 5 minutes was observed. This effect was blocked by superoxide dismutase and catalase. Removal of calcium from the medium or chelating the extracellular calcium with ethyleneglycoldiamine tetraacetate significantly blunted the response to OFRs (p < 0.05). Preincubation of the cells with verapamil did not alter the observed increase in Ca++i. Addition of another divalent cation (Mn++) to the medium partially blocked the rise in calcium levels (p < 0.05). Membrane potential measurements assessed fluorometrically, with the fluorescent probe bisoxonol, demonstrate a transient hyperpolarization of the plasma membrane on exposure to OFRs, temporally associated with the rise in [Ca++]i. In summary, OFRs cause an increase of intracellular calcium in endothelial cells. This response is dependent on extracellular calcium and independent of voltage-sensitive calcium channels. The increase can be partially inhibited by other divalent cations. These results suggest that the transformation of the plasma membrane components (lipid peroxidation and cross-linking of proteins) caused by OFRs may produce cation ionophores.

Original languageEnglish
Pages (from-to)292-297
Number of pages6
JournalSurgery
Volume108
Issue number2
StatePublished - Jan 1 1990
Externally publishedYes

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Free Radicals
Reactive Oxygen Species
Endothelial Cells
Calcium
Divalent Cations
Cell Membrane
Xanthine Oxidase
Ionophores
Calcium Channels
Membrane Lipids
Verapamil
Fluorescent Dyes
Membrane Potentials
Catalase
Lipid Peroxidation
Superoxide Dismutase
Cations
Homeostasis
Fluorescence
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Franceschi, D., Graham, D., Sarasua, M., & Zollinger, R. M. (1990). Mechanisms of oxygen free radical-induced calcium overload in endothelial cells. Surgery, 108(2), 292-297.

Mechanisms of oxygen free radical-induced calcium overload in endothelial cells. / Franceschi, Dido; Graham, D.; Sarasua, M.; Zollinger, R. M.

In: Surgery, Vol. 108, No. 2, 01.01.1990, p. 292-297.

Research output: Contribution to journalArticle

Franceschi, D, Graham, D, Sarasua, M & Zollinger, RM 1990, 'Mechanisms of oxygen free radical-induced calcium overload in endothelial cells', Surgery, vol. 108, no. 2, pp. 292-297.
Franceschi D, Graham D, Sarasua M, Zollinger RM. Mechanisms of oxygen free radical-induced calcium overload in endothelial cells. Surgery. 1990 Jan 1;108(2):292-297.
Franceschi, Dido ; Graham, D. ; Sarasua, M. ; Zollinger, R. M. / Mechanisms of oxygen free radical-induced calcium overload in endothelial cells. In: Surgery. 1990 ; Vol. 108, No. 2. pp. 292-297.
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