Mechanisms of corticosteroid action on lymphocyte subpopulations. V. Effects of in Vivo hydrocortisone on the circulatory kinetics and function of naturally occurring and mitogen-induced suppressor cells in man

Barton F. Haynes, Paul Katz, Anthony S. Fauci

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The present study evaluated the effect of in vivo administration of hydrocortisone (OHC) to normal subjects on the kinetics and function of naturally occurring and mitogen-induced suppressor cells (SC). It was demonstrated that in vivo OHC abrogates the function of naturally occurring SC and converts normal nonresponder peripheral blood (PB) cells to responder status. However, 4 hr following OHC administration at the point of maximal lymphocytopenia, the cells remaining in the circulation could still be activated by Con A to suppress. In addition, unstimulated cells obtained 4 hr after OHC administration markedly enhanced PFC responses when cocultured with fresh autologous cells. It was demonstrated that this enhanced PFC response was independent of 48-hr incubation, monocyte depletion, or changes in B-cell numbers and most likely represented a manifestation of in vivo OHC-induced changes in the circulatory kinetics of immunoregulatory cell populations.

Original languageEnglish
Pages (from-to)169-178
Number of pages10
JournalCellular Immunology
Volume44
Issue number1
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

Fingerprint

Lymphocyte Subsets
Mitogens
Hydrocortisone
Adrenal Cortex Hormones
Lymphopenia
Monocytes
Blood Cells
B-Lymphocytes
Cell Count
Population

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

@article{b378dd7f4ff24c5ba23b1349e2e55904,
title = "Mechanisms of corticosteroid action on lymphocyte subpopulations. V. Effects of in Vivo hydrocortisone on the circulatory kinetics and function of naturally occurring and mitogen-induced suppressor cells in man",
abstract = "The present study evaluated the effect of in vivo administration of hydrocortisone (OHC) to normal subjects on the kinetics and function of naturally occurring and mitogen-induced suppressor cells (SC). It was demonstrated that in vivo OHC abrogates the function of naturally occurring SC and converts normal nonresponder peripheral blood (PB) cells to responder status. However, 4 hr following OHC administration at the point of maximal lymphocytopenia, the cells remaining in the circulation could still be activated by Con A to suppress. In addition, unstimulated cells obtained 4 hr after OHC administration markedly enhanced PFC responses when cocultured with fresh autologous cells. It was demonstrated that this enhanced PFC response was independent of 48-hr incubation, monocyte depletion, or changes in B-cell numbers and most likely represented a manifestation of in vivo OHC-induced changes in the circulatory kinetics of immunoregulatory cell populations.",
author = "Haynes, {Barton F.} and Paul Katz and Fauci, {Anthony S.}",
year = "1979",
month = "1",
day = "1",
doi = "10.1016/0008-8749(79)90037-6",
language = "English",
volume = "44",
pages = "169--178",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Mechanisms of corticosteroid action on lymphocyte subpopulations. V. Effects of in Vivo hydrocortisone on the circulatory kinetics and function of naturally occurring and mitogen-induced suppressor cells in man

AU - Haynes, Barton F.

AU - Katz, Paul

AU - Fauci, Anthony S.

PY - 1979/1/1

Y1 - 1979/1/1

N2 - The present study evaluated the effect of in vivo administration of hydrocortisone (OHC) to normal subjects on the kinetics and function of naturally occurring and mitogen-induced suppressor cells (SC). It was demonstrated that in vivo OHC abrogates the function of naturally occurring SC and converts normal nonresponder peripheral blood (PB) cells to responder status. However, 4 hr following OHC administration at the point of maximal lymphocytopenia, the cells remaining in the circulation could still be activated by Con A to suppress. In addition, unstimulated cells obtained 4 hr after OHC administration markedly enhanced PFC responses when cocultured with fresh autologous cells. It was demonstrated that this enhanced PFC response was independent of 48-hr incubation, monocyte depletion, or changes in B-cell numbers and most likely represented a manifestation of in vivo OHC-induced changes in the circulatory kinetics of immunoregulatory cell populations.

AB - The present study evaluated the effect of in vivo administration of hydrocortisone (OHC) to normal subjects on the kinetics and function of naturally occurring and mitogen-induced suppressor cells (SC). It was demonstrated that in vivo OHC abrogates the function of naturally occurring SC and converts normal nonresponder peripheral blood (PB) cells to responder status. However, 4 hr following OHC administration at the point of maximal lymphocytopenia, the cells remaining in the circulation could still be activated by Con A to suppress. In addition, unstimulated cells obtained 4 hr after OHC administration markedly enhanced PFC responses when cocultured with fresh autologous cells. It was demonstrated that this enhanced PFC response was independent of 48-hr incubation, monocyte depletion, or changes in B-cell numbers and most likely represented a manifestation of in vivo OHC-induced changes in the circulatory kinetics of immunoregulatory cell populations.

UR - http://www.scopus.com/inward/record.url?scp=0018757375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018757375&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(79)90037-6

DO - 10.1016/0008-8749(79)90037-6

M3 - Article

VL - 44

SP - 169

EP - 178

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -