Mechanism of hemin inhibition of erythroid cytoplasmic DNA polymerase

John Byrnes, Kathleen M. Downey, Laura Esserman, Antero G. So

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Hemin, which has an important role in the regulation of hemoglobin synthesis, also regulates the activity of cytoplasmic DNA polymerase from erythroid hyperplastic bone marrow cells and reticulocytes. Hemin inhibits DNA synthesis by binding reversibly to the enzyme. Binding assays demonstrated that hemin prevents association and causes dissociation of the DNA-enzyme complex. This is in contrast to inhibitory compounds that specifically interact with DNA such as ethidium bromide and daunomyein which have little or no effect on the DNA polymerase-template complex. Kinetic analysis reveals that hemin inhibition of DNA synthesis is competitive with respect to template and noncompetitive with respect to substrate. The inhibitory effect of hemin can be reversed by subsequent addition of globin, indicating that the inhibition of DNA synthesis by hemin is not due to irreversible inactivation of the enzyme.

Original languageEnglish
Pages (from-to)796-799
Number of pages4
JournalBiochemistry
Volume14
Issue number4
StatePublished - Dec 1 1975

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Hemin
DNA-Directed DNA Polymerase
DNA
Enzymes
Ethidium
Globins
Reticulocytes
Bone Marrow Cells
Assays
Bone
Hemoglobins
Cells
Association reactions
Kinetics
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Byrnes, J., Downey, K. M., Esserman, L., & So, A. G. (1975). Mechanism of hemin inhibition of erythroid cytoplasmic DNA polymerase. Biochemistry, 14(4), 796-799.

Mechanism of hemin inhibition of erythroid cytoplasmic DNA polymerase. / Byrnes, John; Downey, Kathleen M.; Esserman, Laura; So, Antero G.

In: Biochemistry, Vol. 14, No. 4, 01.12.1975, p. 796-799.

Research output: Contribution to journalArticle

Byrnes, J, Downey, KM, Esserman, L & So, AG 1975, 'Mechanism of hemin inhibition of erythroid cytoplasmic DNA polymerase', Biochemistry, vol. 14, no. 4, pp. 796-799.
Byrnes J, Downey KM, Esserman L, So AG. Mechanism of hemin inhibition of erythroid cytoplasmic DNA polymerase. Biochemistry. 1975 Dec 1;14(4):796-799.
Byrnes, John ; Downey, Kathleen M. ; Esserman, Laura ; So, Antero G. / Mechanism of hemin inhibition of erythroid cytoplasmic DNA polymerase. In: Biochemistry. 1975 ; Vol. 14, No. 4. pp. 796-799.
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