Mechanism of cell growth on calcium phosphate particles

Role of cell-mediated dissolution of calcium phosphate matrix

Herman S Cheung, A. J. Tofe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An in vitro model was established to examine the possible role of cell-mediated dissolution of the calcium phosphate matrix in the 'bioreactivity' property of calcium phosphate biomaterial. OsteoGraf/N-300, a porous hydroxyapatite (calcium phosphate) was chosen as the test material, and human fibroblasts were used in the in vitro cell culture. After a week of culture, porous hydroxyapatite granules (250 to 420 μm) were covered with cells. Cells were seen breaking off and endocytosing small (< 10 μm) fragments from the larger granules. Transmission electron microscopy confirmed that significant number of cells growing on the granules contained intracellular calcium phosphate particles. Particulate of dense and porous hydroxyapatite (< 10 μm) induced DNA synthesis in fibroblasts in a dose-related manner and translocation of protein kinase C from the cytoplasm to cell membrane.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalS.T.P. Pharma Sciences
Volume3
Issue number1
StatePublished - Jan 1 1993
Externally publishedYes

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Durapatite
Growth
Fibroblasts
Biocompatible Materials
Endocytosis
Transmission Electron Microscopy
Protein Kinase C
Cytoplasm
Cell Culture Techniques
Cell Count
Cell Membrane
calcium phosphate
DNA
In Vitro Techniques

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Mechanism of cell growth on calcium phosphate particles : Role of cell-mediated dissolution of calcium phosphate matrix. / Cheung, Herman S; Tofe, A. J.

In: S.T.P. Pharma Sciences, Vol. 3, No. 1, 01.01.1993, p. 51-55.

Research output: Contribution to journalArticle

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