An in vitro model was established to examine the possible role of cell-mediated dissolution of the calcium phosphate matrix in the 'bioreactivity' property of calcium phosphate biomaterial. OsteoGraf/N-300, a porous hydroxyapatite (calcium phosphate) was chosen as the test material, and human fibroblasts were used in the in vitro cell culture. After a week of culture, porous hydroxyapatite granules (250 to 420 μm) were covered with cells. Cells were seen breaking off and endocytosing small (< 10 μm) fragments from the larger granules. Transmission electron microscopy confirmed that significant number of cells growing on the granules contained intracellular calcium phosphate particles. Particulate of dense and porous hydroxyapatite (< 10 μm) induced DNA synthesis in fibroblasts in a dose-related manner and translocation of protein kinase C from the cytoplasm to cell membrane.
|Original language||English (US)|
|Number of pages||5|
|Journal||S.T.P. Pharma Sciences|
|State||Published - Jan 1 1993|
ASJC Scopus subject areas
- Pharmaceutical Science