Mechanism of cell growth on calcium phosphate particles: Role of cell-mediated dissolution of calcium phosphate matrix

H. S. Cheung, A. J. Tofe

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

An in vitro model was established to examine the possible role of cell-mediated dissolution of the calcium phosphate matrix in the 'bioreactivity' property of calcium phosphate biomaterial. OsteoGraf/N-300, a porous hydroxyapatite (calcium phosphate) was chosen as the test material, and human fibroblasts were used in the in vitro cell culture. After a week of culture, porous hydroxyapatite granules (250 to 420 μm) were covered with cells. Cells were seen breaking off and endocytosing small (< 10 μm) fragments from the larger granules. Transmission electron microscopy confirmed that significant number of cells growing on the granules contained intracellular calcium phosphate particles. Particulate of dense and porous hydroxyapatite (< 10 μm) induced DNA synthesis in fibroblasts in a dose-related manner and translocation of protein kinase C from the cytoplasm to cell membrane.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalS.T.P. Pharma Sciences
Volume3
Issue number1
StatePublished - Jan 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Pharmaceutical Science

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