Mechanical injury increases bFGF and CNTF mRNA expression in the mouse retina

Wei Cao, Rong Wen, Feng Li, Matthew M. Lavail, Roy H. Steinberg

Research output: Contribution to journalArticle

128 Scopus citations

Abstract

We characterized the survival-factor response of the normal mouse retina to mechanical injury by examining the expression of mRNAs for basic fibroblast growth factor (bFGF), ciliary neurotrophic factor (CNTF), and their receptors, FGE receptor-1 (FGFR-1) and CNTF receptor alpha (CNTFR- alpha). The retina was injured by making an incision through the choroid and retinal pigment epithelium that penetrated the subretinal space of each eye of an adult BALB/c mouse. Retinas were taken 6 hr, 12 hr, 1, 2, 4, 7, 10 and 16 days post-injury. Control animals were without injury. Northern blot analysis was performed to determine bFGF, CNTF and their receptor mRNA levels. A significant increase in bFGF and CNTF mRNAs was observed after injury, along with an increase in glial fibrillary acidic protein (GFAP) expression. More than 2-fold of upregulation of bFGF mRNA was seen as early as 6 hr after injury. This increase reached a maximum of more than 5-fold at day 2 post-injury and then declined slowly, and was still about 2.5-fold of the control level by day 16. Expression of CNTF showed a small increase of about 1.6-fold at 6 hr after injury. The upregulation reached a peak level of about 2.7-fold at day 4 after injury, then declined to control level by day 16. There was only a very small increase in FGFR-1 at 6, 12 and 24 hr after injury, and no significant increases in FGFR-1 at time points longer than 1 day post-injury. Expression of GFAP followed a time course similar to that of bFGP. We conclude that mechanical injury induces bFGF, CNTF, and GFAP expression in the mouse retina with time courses similar to the upregulation of these molecules in rat retina. Compared to the upregulation in rat retina, however, the injury-induced upregulation of bFGF and GFAP is much less in the mouse retina. In addition, there was only a very small induction of FGFR-1 expression in the mouse retina. These findings may explain, at least in part, the lack of injury-induced photoreceptor protection in the mouse retina.

Original languageEnglish (US)
Pages (from-to)241-248
Number of pages8
JournalExperimental Eye Research
Volume65
Issue number2
DOIs
StatePublished - Aug 1997
Externally publishedYes

Keywords

  • Basic FGF
  • CNTF
  • CNTF-alpha
  • FGFR-1
  • GFAP
  • Injury
  • Mouse
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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