Mechanical Chondroplasty: Early Metabolic Consequences In Vitro

Lee Kaplan, Bryan Royce, Brian Meier, Justin M. Hoffmann, Jonathan D. Barlow, Yan Lu, Herman F. Stampfli

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: The purpose of this study was to determine the depth of penetration from mechanical chondroplasty and metabolic consequences of this procedure on the remaining articular cartilage. Methods: Mechanical chondroplasty was performed in vitro on a portion of fresh grade I or II articular cartilage from 8 human knee arthroplasty specimens. Treated and control (untreated) explants (approximately 30 mg) were cut from the cartilage. The explants were divided into 2 groups, day 1 and day 4, placed separately in a 48-well plate containing media, and incubated at 37°C for 24 hours. After the 24-hour incubation, the explants were weighed on day 1 and day 4, and explant media were removed and tested for total proteoglycan synthesis and aggrecan synthesis. At time 0, 2 sets (2.6 mm each) of treated and control cartilage slices were cut with a precision saw. One set was stained for confocal laser microscopy via a cytotoxicity stain to determine cell viability. The second set was stained with H&E to determine depth of penetration. Results: The mean depth of penetration was 252.8 ± 78 μm. There was no significant difference (P > .25) between total proteoglycan synthesis for control versus treatment groups on day 1 or 4. Aggrecan synthesis was significantly reduced on day 1 when normalized for tissue weight (P = .019) and double-stranded deoxyribonucleic acid (P = .004). On day 4, no significant difference was detected. Confocal laser microscopy did not show cell death below the zone of treatment. Conclusions: There was no significant metabolic consequence caused by chondroplasty to the remaining articular cartilage, and the zone of injury was limited to the treatment area. Clinical Relevance: Mechanical chondroplasty causes no significant metabolic consequences to articular cartilage under these conditions.

Original languageEnglish
Pages (from-to)923-929
Number of pages7
JournalArthroscopy - Journal of Arthroscopic and Related Surgery
Volume23
Issue number9
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

Fingerprint

Articular Cartilage
Confocal Microscopy
Aggrecans
Proteoglycans
Cartilage
Knee Replacement Arthroplasties
Cell Survival
Cell Death
Coloring Agents
Therapeutics
Weights and Measures
In Vitro Techniques
DNA
Wounds and Injuries

Keywords

  • Articular cartilage
  • Cartilage defects
  • Debridement
  • Knee
  • Mechanical chondroplasty
  • Osteoarthritis

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Mechanical Chondroplasty : Early Metabolic Consequences In Vitro. / Kaplan, Lee; Royce, Bryan; Meier, Brian; Hoffmann, Justin M.; Barlow, Jonathan D.; Lu, Yan; Stampfli, Herman F.

In: Arthroscopy - Journal of Arthroscopic and Related Surgery, Vol. 23, No. 9, 01.09.2007, p. 923-929.

Research output: Contribution to journalArticle

Kaplan, Lee ; Royce, Bryan ; Meier, Brian ; Hoffmann, Justin M. ; Barlow, Jonathan D. ; Lu, Yan ; Stampfli, Herman F. / Mechanical Chondroplasty : Early Metabolic Consequences In Vitro. In: Arthroscopy - Journal of Arthroscopic and Related Surgery. 2007 ; Vol. 23, No. 9. pp. 923-929.
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abstract = "Purpose: The purpose of this study was to determine the depth of penetration from mechanical chondroplasty and metabolic consequences of this procedure on the remaining articular cartilage. Methods: Mechanical chondroplasty was performed in vitro on a portion of fresh grade I or II articular cartilage from 8 human knee arthroplasty specimens. Treated and control (untreated) explants (approximately 30 mg) were cut from the cartilage. The explants were divided into 2 groups, day 1 and day 4, placed separately in a 48-well plate containing media, and incubated at 37°C for 24 hours. After the 24-hour incubation, the explants were weighed on day 1 and day 4, and explant media were removed and tested for total proteoglycan synthesis and aggrecan synthesis. At time 0, 2 sets (2.6 mm each) of treated and control cartilage slices were cut with a precision saw. One set was stained for confocal laser microscopy via a cytotoxicity stain to determine cell viability. The second set was stained with H&E to determine depth of penetration. Results: The mean depth of penetration was 252.8 ± 78 μm. There was no significant difference (P > .25) between total proteoglycan synthesis for control versus treatment groups on day 1 or 4. Aggrecan synthesis was significantly reduced on day 1 when normalized for tissue weight (P = .019) and double-stranded deoxyribonucleic acid (P = .004). On day 4, no significant difference was detected. Confocal laser microscopy did not show cell death below the zone of treatment. Conclusions: There was no significant metabolic consequence caused by chondroplasty to the remaining articular cartilage, and the zone of injury was limited to the treatment area. Clinical Relevance: Mechanical chondroplasty causes no significant metabolic consequences to articular cartilage under these conditions.",
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