Measuring the diaspora for virus-specific CD8+ T cells

Dana R. Marshall, Stephen J. Turner, Gabrielle T. Belz, Suzette Wingo, Samita Andreansky, Mark Y. Sangster, Janice M. Riberdy, Tiebin Liu, Ming Tan, Peter C. Doherty

Research output: Contribution to journalArticle

238 Citations (Scopus)

Abstract

The CD8+ T cell diaspora has been analyzed after secondary challenge with an influenza A virus that replicates only in the respiratory tract. Numbers of DbNP366-and DbPA224-specific CD8+ T cells were measured by tetramer staining at the end of the recall response, then followed sequentially in the lung, lymph nodes, spleen, blood, and other organs. The extent of clonal expansion did not reflect the sizes of the preexisting memory T cell pools. Although the high-frequency CD8+ tetramer+ populations in the pneumonic lung and mediastinal lymph nodes fell rapidly from peak values, the "whole mouse" virus-specific CD8+ T cell counts decreased only 2-fold over the 4 weeks after infection, then subsided at a fairly steady rate to reach a plateau at about 2 months. The largest numbers were found throughout in the spleen, then the bone marrow. The CD8+DbNP366+ and CD8+DbPA224+ sets remained significantly enlarged for at least 4 months, declining at equivalent rates while retaining the nucleoprotein > acid polymerase immunodominance hierarchy characteristic of the earlier antigen-driven phase. Lowest levels of the CD69 "activation marker" were detected consistently on virus-specific CD8+ T cells in the blood, then the spleen. Those in the bone marrow and liver were intermediate, and CD69hi T cells were very prominent in the regional lymph nodes and the nasal-associated lymphoid tissue. Any population of "resting" CD8+ memory T cells is thus phenotypically heterogeneous, widely dispersed, and subject to broad homeostatic and local environmental effects irrespective of epitope specificity or magnitude.

Original languageEnglish
Pages (from-to)6313-6318
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number11
DOIs
StatePublished - May 22 2001
Externally publishedYes

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Viruses
T-Lymphocytes
Spleen
Lymph Nodes
Lung
Bone Marrow
Nucleoproteins
Influenza A virus
Lymphoid Tissue
Nose
Respiratory System
Population
Epitopes
Cell Count
Staining and Labeling
Antigens
Acids
Liver
Infection

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Measuring the diaspora for virus-specific CD8+ T cells. / Marshall, Dana R.; Turner, Stephen J.; Belz, Gabrielle T.; Wingo, Suzette; Andreansky, Samita; Sangster, Mark Y.; Riberdy, Janice M.; Liu, Tiebin; Tan, Ming; Doherty, Peter C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 11, 22.05.2001, p. 6313-6318.

Research output: Contribution to journalArticle

Marshall, DR, Turner, SJ, Belz, GT, Wingo, S, Andreansky, S, Sangster, MY, Riberdy, JM, Liu, T, Tan, M & Doherty, PC 2001, 'Measuring the diaspora for virus-specific CD8+ T cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 11, pp. 6313-6318. https://doi.org/10.1073/pnas.101132698
Marshall, Dana R. ; Turner, Stephen J. ; Belz, Gabrielle T. ; Wingo, Suzette ; Andreansky, Samita ; Sangster, Mark Y. ; Riberdy, Janice M. ; Liu, Tiebin ; Tan, Ming ; Doherty, Peter C. / Measuring the diaspora for virus-specific CD8+ T cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 11. pp. 6313-6318.
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