Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells

Hendrik Kindmark, Martin Köhler, Thomas Nilsson, Per Arkhammar, Karl Ludvig Wiechel, Patrik Rorsman, Suad Efendić, Per Olaf Berggren

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration ([Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extracellular ATP produced a transient rapid rise in [Ca2+]i. Oscillations in [Ca2+]i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutamide with increases in [Ca2+]i, whereas the sulphonamide diazoxide caused a decrease in [Ca2+]i. These findings confirm previous results obtained in rodent β-cells.

Original languageEnglish (US)
Pages (from-to)310-314
Number of pages5
JournalFEBS letters
Volume291
Issue number2
DOIs
StatePublished - Oct 21 1991

Keywords

  • Cytoplasmic free Ca concentration
  • Fura-2
  • Human insulinoma
  • Human pancreatic islet

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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