Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells

Hendrik Kindmark, Martin Köhler, Thomas Nilsson, Per Arkhammar, Karl Ludvig Wiechel, Patrik Rorsman, Suad Efendić, Per Olaf Berggren

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration ([Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extracellular ATP produced a transient rapid rise in [Ca2+]i. Oscillations in [Ca2+]i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutamide with increases in [Ca2+]i, whereas the sulphonamide diazoxide caused a decrease in [Ca2+]i. These findings confirm previous results obtained in rodent β-cells.

Original languageEnglish
Pages (from-to)310-314
Number of pages5
JournalFEBS Letters
Volume291
Issue number2
DOIs
StatePublished - Oct 21 1991
Externally publishedYes

Fingerprint

Insulinoma
Islets of Langerhans
Tolbutamide
Glucose
Diazoxide
Sulfonamides
Depolarization
Sugars
Rodentia
Adenosine Triphosphate

Keywords

  • Cytoplasmic free Ca concentration
  • Fura-2
  • Human insulinoma
  • Human pancreatic islet

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kindmark, H., Köhler, M., Nilsson, T., Arkhammar, P., Wiechel, K. L., Rorsman, P., ... Berggren, P. O. (1991). Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells. FEBS Letters, 291(2), 310-314. https://doi.org/10.1016/0014-5793(91)81309-V

Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells. / Kindmark, Hendrik; Köhler, Martin; Nilsson, Thomas; Arkhammar, Per; Wiechel, Karl Ludvig; Rorsman, Patrik; Efendić, Suad; Berggren, Per Olaf.

In: FEBS Letters, Vol. 291, No. 2, 21.10.1991, p. 310-314.

Research output: Contribution to journalArticle

Kindmark, H, Köhler, M, Nilsson, T, Arkhammar, P, Wiechel, KL, Rorsman, P, Efendić, S & Berggren, PO 1991, 'Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells', FEBS Letters, vol. 291, no. 2, pp. 310-314. https://doi.org/10.1016/0014-5793(91)81309-V
Kindmark H, Köhler M, Nilsson T, Arkhammar P, Wiechel KL, Rorsman P et al. Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells. FEBS Letters. 1991 Oct 21;291(2):310-314. https://doi.org/10.1016/0014-5793(91)81309-V
Kindmark, Hendrik ; Köhler, Martin ; Nilsson, Thomas ; Arkhammar, Per ; Wiechel, Karl Ludvig ; Rorsman, Patrik ; Efendić, Suad ; Berggren, Per Olaf. / Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells. In: FEBS Letters. 1991 ; Vol. 291, No. 2. pp. 310-314.
@article{da848abdf401437d95c377e79a02514e,
title = "Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells",
abstract = "In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration ([Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extracellular ATP produced a transient rapid rise in [Ca2+]i. Oscillations in [Ca2+]i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutamide with increases in [Ca2+]i, whereas the sulphonamide diazoxide caused a decrease in [Ca2+]i. These findings confirm previous results obtained in rodent β-cells.",
keywords = "Cytoplasmic free Ca concentration, Fura-2, Human insulinoma, Human pancreatic islet",
author = "Hendrik Kindmark and Martin K{\"o}hler and Thomas Nilsson and Per Arkhammar and Wiechel, {Karl Ludvig} and Patrik Rorsman and Suad Efendić and Berggren, {Per Olaf}",
year = "1991",
month = "10",
day = "21",
doi = "10.1016/0014-5793(91)81309-V",
language = "English",
volume = "291",
pages = "310--314",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells

AU - Kindmark, Hendrik

AU - Köhler, Martin

AU - Nilsson, Thomas

AU - Arkhammar, Per

AU - Wiechel, Karl Ludvig

AU - Rorsman, Patrik

AU - Efendić, Suad

AU - Berggren, Per Olaf

PY - 1991/10/21

Y1 - 1991/10/21

N2 - In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration ([Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extracellular ATP produced a transient rapid rise in [Ca2+]i. Oscillations in [Ca2+]i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutamide with increases in [Ca2+]i, whereas the sulphonamide diazoxide caused a decrease in [Ca2+]i. These findings confirm previous results obtained in rodent β-cells.

AB - In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration ([Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extracellular ATP produced a transient rapid rise in [Ca2+]i. Oscillations in [Ca2+]i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutamide with increases in [Ca2+]i, whereas the sulphonamide diazoxide caused a decrease in [Ca2+]i. These findings confirm previous results obtained in rodent β-cells.

KW - Cytoplasmic free Ca concentration

KW - Fura-2

KW - Human insulinoma

KW - Human pancreatic islet

UR - http://www.scopus.com/inward/record.url?scp=0025941839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025941839&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(91)81309-V

DO - 10.1016/0014-5793(91)81309-V

M3 - Article

VL - 291

SP - 310

EP - 314

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -