Measurement variability of the bulbar conjunctival microvasculature in healthy subjects using functional slit lamp biomicroscopy (FSLB)

Zhe Xu, Hong Jiang, Aizhu Tao, Shuangqing Wu, Wentao Yan, Jin Yuan, Che Liu, Delia Cabrera DeBuc, Jianhua Wang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The goal was to determine the variability of the quantitative measurement of the bulbar conjunctival microvascular morphology and hemodynamics by testing the repeatability and variation during office hours. Functional slit-lamp biomicroscopy (FSLB) was used to image the bulbar conjunctival microvasculature, including the vessel diameter, blood flow velocity/rate and fractal dimensions of the microvascular network. The temporal side of the bulbar conjunctiva in 20 healthy subjects was imaged. The subject was imaged at 9 AM to test the measurement repeatability by two independent graders. The intraclass correlation coefficient (ICC) and coefficient of repeatability (CoR) were calculated. These same subjects were then imaged every two hours from 9 AM to 5 PM to test the variation during office hours. Custom software was used to semi-automatically process all measurements. The CoR% and ICC values between two graders for measuring the vessel diameter were 4.87% and 0.989, respectively. For the axial blood flow velocity, the CoR% and ICC were 11.49% and 0.997, respectively. From 9 AM to 5 PM, there were no significant variations in the vessel diameter and hemodynamics (P> 0.05), whereas the fractal dimensions of the non-invasive microvascular perfusion maps (nMPMs) were significantly increased at 3 PM and 5 PM compared with the baseline obtained at 9 AM (P< 0.05). FSLB appears to be capable of measuring vessel diameter, blood flow velocity and fractal dimension of the microvascular network in the bulbar conjunctiva. Slight variations over office hours were observed in the microvascular network, while the blood flow velocity remained stable.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalMicrovascular Research
Volume101
DOIs
StatePublished - Sep 1 2015

Fingerprint

Blood Flow Velocity
Microvessels
Electric lamps
Flow velocity
Fractals
Healthy Volunteers
Fractal dimension
Conjunctiva
Blood vessels
Hemodynamics
Blood
Software
Perfusion
Slit Lamp
Testing

Keywords

  • Bulbar conjunctival microvascular morphology
  • Diurnal variation during office hours
  • Functional slit lamp biomicroscopy (FSLB)
  • Hemodynamics
  • Repeatability

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Measurement variability of the bulbar conjunctival microvasculature in healthy subjects using functional slit lamp biomicroscopy (FSLB). / Xu, Zhe; Jiang, Hong; Tao, Aizhu; Wu, Shuangqing; Yan, Wentao; Yuan, Jin; Liu, Che; Cabrera DeBuc, Delia; Wang, Jianhua.

In: Microvascular Research, Vol. 101, 01.09.2015, p. 15-19.

Research output: Contribution to journalArticle

@article{493ab7faac9b4057967d8e0afceee920,
title = "Measurement variability of the bulbar conjunctival microvasculature in healthy subjects using functional slit lamp biomicroscopy (FSLB)",
abstract = "The goal was to determine the variability of the quantitative measurement of the bulbar conjunctival microvascular morphology and hemodynamics by testing the repeatability and variation during office hours. Functional slit-lamp biomicroscopy (FSLB) was used to image the bulbar conjunctival microvasculature, including the vessel diameter, blood flow velocity/rate and fractal dimensions of the microvascular network. The temporal side of the bulbar conjunctiva in 20 healthy subjects was imaged. The subject was imaged at 9 AM to test the measurement repeatability by two independent graders. The intraclass correlation coefficient (ICC) and coefficient of repeatability (CoR) were calculated. These same subjects were then imaged every two hours from 9 AM to 5 PM to test the variation during office hours. Custom software was used to semi-automatically process all measurements. The CoR{\%} and ICC values between two graders for measuring the vessel diameter were 4.87{\%} and 0.989, respectively. For the axial blood flow velocity, the CoR{\%} and ICC were 11.49{\%} and 0.997, respectively. From 9 AM to 5 PM, there were no significant variations in the vessel diameter and hemodynamics (P> 0.05), whereas the fractal dimensions of the non-invasive microvascular perfusion maps (nMPMs) were significantly increased at 3 PM and 5 PM compared with the baseline obtained at 9 AM (P< 0.05). FSLB appears to be capable of measuring vessel diameter, blood flow velocity and fractal dimension of the microvascular network in the bulbar conjunctiva. Slight variations over office hours were observed in the microvascular network, while the blood flow velocity remained stable.",
keywords = "Bulbar conjunctival microvascular morphology, Diurnal variation during office hours, Functional slit lamp biomicroscopy (FSLB), Hemodynamics, Repeatability",
author = "Zhe Xu and Hong Jiang and Aizhu Tao and Shuangqing Wu and Wentao Yan and Jin Yuan and Che Liu and {Cabrera DeBuc}, Delia and Jianhua Wang",
year = "2015",
month = "9",
day = "1",
doi = "10.1016/j.mvr.2015.05.003",
language = "English (US)",
volume = "101",
pages = "15--19",
journal = "Microvascular Research",
issn = "0026-2862",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Measurement variability of the bulbar conjunctival microvasculature in healthy subjects using functional slit lamp biomicroscopy (FSLB)

AU - Xu, Zhe

AU - Jiang, Hong

AU - Tao, Aizhu

AU - Wu, Shuangqing

AU - Yan, Wentao

AU - Yuan, Jin

AU - Liu, Che

AU - Cabrera DeBuc, Delia

AU - Wang, Jianhua

PY - 2015/9/1

Y1 - 2015/9/1

N2 - The goal was to determine the variability of the quantitative measurement of the bulbar conjunctival microvascular morphology and hemodynamics by testing the repeatability and variation during office hours. Functional slit-lamp biomicroscopy (FSLB) was used to image the bulbar conjunctival microvasculature, including the vessel diameter, blood flow velocity/rate and fractal dimensions of the microvascular network. The temporal side of the bulbar conjunctiva in 20 healthy subjects was imaged. The subject was imaged at 9 AM to test the measurement repeatability by two independent graders. The intraclass correlation coefficient (ICC) and coefficient of repeatability (CoR) were calculated. These same subjects were then imaged every two hours from 9 AM to 5 PM to test the variation during office hours. Custom software was used to semi-automatically process all measurements. The CoR% and ICC values between two graders for measuring the vessel diameter were 4.87% and 0.989, respectively. For the axial blood flow velocity, the CoR% and ICC were 11.49% and 0.997, respectively. From 9 AM to 5 PM, there were no significant variations in the vessel diameter and hemodynamics (P> 0.05), whereas the fractal dimensions of the non-invasive microvascular perfusion maps (nMPMs) were significantly increased at 3 PM and 5 PM compared with the baseline obtained at 9 AM (P< 0.05). FSLB appears to be capable of measuring vessel diameter, blood flow velocity and fractal dimension of the microvascular network in the bulbar conjunctiva. Slight variations over office hours were observed in the microvascular network, while the blood flow velocity remained stable.

AB - The goal was to determine the variability of the quantitative measurement of the bulbar conjunctival microvascular morphology and hemodynamics by testing the repeatability and variation during office hours. Functional slit-lamp biomicroscopy (FSLB) was used to image the bulbar conjunctival microvasculature, including the vessel diameter, blood flow velocity/rate and fractal dimensions of the microvascular network. The temporal side of the bulbar conjunctiva in 20 healthy subjects was imaged. The subject was imaged at 9 AM to test the measurement repeatability by two independent graders. The intraclass correlation coefficient (ICC) and coefficient of repeatability (CoR) were calculated. These same subjects were then imaged every two hours from 9 AM to 5 PM to test the variation during office hours. Custom software was used to semi-automatically process all measurements. The CoR% and ICC values between two graders for measuring the vessel diameter were 4.87% and 0.989, respectively. For the axial blood flow velocity, the CoR% and ICC were 11.49% and 0.997, respectively. From 9 AM to 5 PM, there were no significant variations in the vessel diameter and hemodynamics (P> 0.05), whereas the fractal dimensions of the non-invasive microvascular perfusion maps (nMPMs) were significantly increased at 3 PM and 5 PM compared with the baseline obtained at 9 AM (P< 0.05). FSLB appears to be capable of measuring vessel diameter, blood flow velocity and fractal dimension of the microvascular network in the bulbar conjunctiva. Slight variations over office hours were observed in the microvascular network, while the blood flow velocity remained stable.

KW - Bulbar conjunctival microvascular morphology

KW - Diurnal variation during office hours

KW - Functional slit lamp biomicroscopy (FSLB)

KW - Hemodynamics

KW - Repeatability

UR - http://www.scopus.com/inward/record.url?scp=84938894132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938894132&partnerID=8YFLogxK

U2 - 10.1016/j.mvr.2015.05.003

DO - 10.1016/j.mvr.2015.05.003

M3 - Article

VL - 101

SP - 15

EP - 19

JO - Microvascular Research

JF - Microvascular Research

SN - 0026-2862

ER -