Abstract
We report on studies aimed at employing the atomic force microscope (AFM) to measure the viscosity of aqueous solutions. At ambient temperature, the AFM cantilever undergoes thermal fluctuations that are highly sensitive to the local environment. Here, we present measurements of the cantilever's resonant frequency in aqueous solutions of glycerol, sucrose, ethanol, sodium chloride, polyethylene glycol, and bovine plasma albumin. The measurements revealed that variations in the resonant frequency of the cantilever in the different solutions are largely dependent on the viscosity of the medium. An application of this technique is to monitor the progression of a chemical reaction where a change in viscosity is expected to occur. An example is demonstrated through monitoring of the hydrolysis of double stranded deoxyribonucleic acid by DNase I.
| Original language | English |
|---|---|
| Pages (from-to) | 2731-2734 |
| Number of pages | 4 |
| Journal | Review of Scientific Instruments |
| Volume | 72 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 2001 |
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ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)
- Instrumentation
Cite this
Measurement of solution viscosity by atomic force microscopy. / Ahmed, Nabil; Nino, Diego F.; Moy, Vincent T.
In: Review of Scientific Instruments, Vol. 72, No. 6, 01.06.2001, p. 2731-2734.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement of solution viscosity by atomic force microscopy
AU - Ahmed, Nabil
AU - Nino, Diego F.
AU - Moy, Vincent T.
PY - 2001/6/1
Y1 - 2001/6/1
N2 - We report on studies aimed at employing the atomic force microscope (AFM) to measure the viscosity of aqueous solutions. At ambient temperature, the AFM cantilever undergoes thermal fluctuations that are highly sensitive to the local environment. Here, we present measurements of the cantilever's resonant frequency in aqueous solutions of glycerol, sucrose, ethanol, sodium chloride, polyethylene glycol, and bovine plasma albumin. The measurements revealed that variations in the resonant frequency of the cantilever in the different solutions are largely dependent on the viscosity of the medium. An application of this technique is to monitor the progression of a chemical reaction where a change in viscosity is expected to occur. An example is demonstrated through monitoring of the hydrolysis of double stranded deoxyribonucleic acid by DNase I.
AB - We report on studies aimed at employing the atomic force microscope (AFM) to measure the viscosity of aqueous solutions. At ambient temperature, the AFM cantilever undergoes thermal fluctuations that are highly sensitive to the local environment. Here, we present measurements of the cantilever's resonant frequency in aqueous solutions of glycerol, sucrose, ethanol, sodium chloride, polyethylene glycol, and bovine plasma albumin. The measurements revealed that variations in the resonant frequency of the cantilever in the different solutions are largely dependent on the viscosity of the medium. An application of this technique is to monitor the progression of a chemical reaction where a change in viscosity is expected to occur. An example is demonstrated through monitoring of the hydrolysis of double stranded deoxyribonucleic acid by DNase I.
UR - http://www.scopus.com/inward/record.url?scp=0035365066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035365066&partnerID=8YFLogxK
U2 - 10.1063/1.1368856
DO - 10.1063/1.1368856
M3 - Article
AN - SCOPUS:0035365066
VL - 72
SP - 2731
EP - 2734
JO - Review of Scientific Instruments
JF - Review of Scientific Instruments
SN - 0034-6748
IS - 6
ER -