Measurement of solution viscosity by atomic force microscopy

Nabil Ahmed, Diego F. Nino, Vincent T. Moy

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

We report on studies aimed at employing the atomic force microscope (AFM) to measure the viscosity of aqueous solutions. At ambient temperature, the AFM cantilever undergoes thermal fluctuations that are highly sensitive to the local environment. Here, we present measurements of the cantilever's resonant frequency in aqueous solutions of glycerol, sucrose, ethanol, sodium chloride, polyethylene glycol, and bovine plasma albumin. The measurements revealed that variations in the resonant frequency of the cantilever in the different solutions are largely dependent on the viscosity of the medium. An application of this technique is to monitor the progression of a chemical reaction where a change in viscosity is expected to occur. An example is demonstrated through monitoring of the hydrolysis of double stranded deoxyribonucleic acid by DNase I.

Original languageEnglish
Pages (from-to)2731-2734
Number of pages4
JournalReview of Scientific Instruments
Volume72
Issue number6
DOIs
StatePublished - Jun 1 2001

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Atomic force microscopy
atomic force microscopy
Viscosity
viscosity
resonant frequencies
Natural frequencies
Microscopes
microscopes
aqueous solutions
sucrose
Sugar (sucrose)
sodium chlorides
Sodium chloride
glycerols
albumins
Glycerol
progressions
Polyethylene glycols
ambient temperature
hydrolysis

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)
  • Instrumentation

Cite this

Measurement of solution viscosity by atomic force microscopy. / Ahmed, Nabil; Nino, Diego F.; Moy, Vincent T.

In: Review of Scientific Instruments, Vol. 72, No. 6, 01.06.2001, p. 2731-2734.

Research output: Contribution to journalArticle

Ahmed, Nabil ; Nino, Diego F. ; Moy, Vincent T. / Measurement of solution viscosity by atomic force microscopy. In: Review of Scientific Instruments. 2001 ; Vol. 72, No. 6. pp. 2731-2734.
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