Measurement of creatine kinase reaction rate in human brain using magnetization transfer image-selected in vivo spectroscopy (MT-ISIS) and a volume 31P/ 1H radiofrequency coil in a clinical 3-T MRI system

Eun Kee Jeong, Young Hoon Sung, Seong Eun Kim, Chun Zuo, Xianfeng Shi, Eric A. Mellon, Perry F. Renshaw

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


High-energy phosphate metabolism, which allows the synthesis and regeneration of adenosine triphosphate (ATP), is a vital process for neuronal survival and activity. In particular, creatine kinase (CK) serves as an energy reservoir for the rapid buffering of ATP levels. Altered CK enzyme activity, reflecting compromised high-energy phosphate metabolism or mitochondrial dysfunction in the brain, can be assessed using magnetization transfer (MT) MRS. MT 31P MRS has been used to measure the forward CK reaction rate in animal and human brain, employing a surface radiofrequency coil. However, long acquisition times and excessive radiofrequency irradiation prevent these methods from being used routinely for clinical evaluations. In this article, a new MT 31P MRS method is presented, which can be practically used to measure the CK forward reaction rate constant in a clinical MRI system employing a volume head 31P coil for spatial localization, without contamination from the scalp muscle, and an acquisition time of 30min. Other advantages associated with the method include radiofrequency homogeneity within the regions of interest of the brain using a volume coil with image-selected in vivo spectroscopy localization, and reduction of the specific absorption rate using nonadiabatic radiofrequency pulses for MT saturation. The mean value of k f was measured as 0.320±0.075s -1 from 10 healthy volunteers with an age range of 18-40 years. These values are consistent with those obtained using earlier methods, and the technique may be used routinely to evaluate energetic processes in the brain on a clinical MRI system.

Original languageEnglish (US)
Pages (from-to)765-770
Number of pages6
JournalNMR in biomedicine
Issue number7
StatePublished - Aug 2011
Externally publishedYes


  • Creatine-Kinase
  • ISIS
  • Magnetization-transfer 31P MRS
  • MT-31P-MRS

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy


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