Matrix metalloproteinase expression during mouse peri-implantation development

Mark E Sharkey, Richard R. Adler, Gary L. Nieder, Carol A. Brenner

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

PROBLEM: The purpose of this study was to define the temporal expression and to quantitate the mRNA levels of collagenase, 72 kDa, 92 kDa, and membrane-type matrix metalloproteinases during the peri-implantation period of pregnancy in the mouse uterus. Embryonic expression of 72 kDa and 92 kDa matrix metalloproteinases, as well as interleukin 1α, was also investigated. METHODS: Uterine matrix metalloproteinases were detected using gelatin substrate gel electrophoresis (zymography) and reverse-transcription polymerase chain reaction methodology was used to detect and quantitate different mRNA species in the mouse uterus and blastocyst. RESULTS: Collagenase, 72 kDa, and 92 kDa matrix metalloproteinases are developmentally regulated during the peri-implantation period of pregnancy, but membrane-type matrix metalloproteinase appears to be expressed constitutively. Matrix metalloproteinase mRNA levels have been quantitated and confirm the observed developmental expression patterns. Prominent expression of both 92 kDa matrix metalloproteinase and interleukin 1α was observed in blastocysts during outgrowth while weak expression of the 72 kDa matrix metalloproteinase was detected. CONCLUSIONS: The data provide evidence of matrix metalloproteinase expression in vivo and substantiate their potential role in tissue remodeling prior to and during blastocyst implantation. Expression of interleukin 1α, 92 kDa, and 72 kDa matrix metalloproteinases suggests that these proteins are important for trophoblast invasion associated with implantation of the early embryo.

Original languageEnglish
Pages (from-to)72-80
Number of pages9
JournalAmerican Journal of Reproductive Immunology
Volume36
Issue number2
StatePublished - Aug 1 1996
Externally publishedYes

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Matrix Metalloproteinases
Membrane-Associated Matrix Metalloproteinases
Interleukin-1
Matrix Metalloproteinase 1
Blastocyst
Collagenases
Messenger RNA
Uterus
Pregnancy
Trophoblasts
Gelatin
Reverse Transcription
Electrophoresis
Gels
Polymerase Chain Reaction
Proteins

Keywords

  • Endometrium
  • Implantation
  • Matrix metalloproteinases
  • Mouse embryos
  • Uterus

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Matrix metalloproteinase expression during mouse peri-implantation development. / Sharkey, Mark E; Adler, Richard R.; Nieder, Gary L.; Brenner, Carol A.

In: American Journal of Reproductive Immunology, Vol. 36, No. 2, 01.08.1996, p. 72-80.

Research output: Contribution to journalArticle

Sharkey, Mark E ; Adler, Richard R. ; Nieder, Gary L. ; Brenner, Carol A. / Matrix metalloproteinase expression during mouse peri-implantation development. In: American Journal of Reproductive Immunology. 1996 ; Vol. 36, No. 2. pp. 72-80.
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