Maternal Roux-en-Y gastric bypass impairs insulin action and endocrine pancreatic function in male F1 offspring

Carla Bruna Pietrobon, Iala Milene Bertasso, Rosane Aparecida Ribeiro, Ana Claudia Paiva Alegre-Maller, Camila Lubaczeuski, Antonio Carlos Boschero, Allan Cezar Faria Araújo, Sandra Lucinei Balbo, Maria Lúcia Bonfleur

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Purpose: Obesity is predominant in women of reproductive age. Roux-en-Y gastric bypass (RYGB) is the most common bariatric procedure that is performed in obese women for weight loss and metabolic improvement. However, some studies suggest that this procedure negatively affects offspring. Herein, using Western diet (WD)-obese female rats, we investigated the effects of maternal RYGB on postnatal body development, glucose tolerance, insulin secretion and action in their adult male F1 offspring. Methods: Female Wistar rats consumed a Western diet (WD) for 18 weeks, before being submitted to RYGB (WD-RYGB) or SHAM (WD-SHAM) operations. After 5 weeks, WD-RYGB and WD-SHAM females were mated with control male breeders, and the F1 offspring were identified as: WD-RYGB-F1 and WD-SHAM-F1. Results: The male F1 offspring of WD-RYGB dams exhibited decreased BW, but enhanced total nasoanal length gain. At 120 days of age, WD-RYGB-F1 rats displayed normal fasting glycemia and glucose tolerance but demonstrated reduced insulinemia and higher glucose disappearance after insulin stimulus. In addition, these rodents presented insulin resistance in the gastrocnemius muscle and retroperitoneal fat, as judged by lower Akt phosphorylation after insulin administration, but an increase in this protein in the liver. Finally, the islets from WD-RYGB-F1 rats secreted less insulin in response to glucose and displayed increased β-cell area and mass. Conclusions: RYGB in WD dams negatively affected their F1 offspring, leading to catch-up growth, insulin resistance in skeletal muscle and white fat, and β-cell dysfunction. Therefore, our data are the first to demonstrate that the RYGB in female rats may aggravate the metabolic imprinting induced by maternal WD consumption, in their male F1 descendants. However, since we only used male F1 rats, further studies are necessary to demonstrate if such effect may also occur in female F1 offspring from dams that underwent RYGB operation.

Original languageEnglish (US)
Pages (from-to)1067-1079
Number of pages13
JournalEuropean Journal of Nutrition
Volume59
Issue number3
DOIs
StatePublished - Apr 1 2020
Externally publishedYes

Keywords

  • Bariatric operation
  • Insulin resistance
  • Insulin secretion
  • Maternal programming
  • Metabolic imprinting
  • Obesity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Fingerprint

Dive into the research topics of 'Maternal Roux-en-Y gastric bypass impairs insulin action and endocrine pancreatic function in male F1 offspring'. Together they form a unique fingerprint.

Cite this