Maternal Dead-End1 is required for vegetal cortical microtubule assembly during Xenopus axis specification

Wenyan Mei, Zhigang Jin, Fangfang Lai, Tyler Schwend, Douglas W. Houston, Mary Lou L King, Jing Yang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Vertebrate axis specification is an evolutionarily conserved developmental process that relies on asymmetric activation of Wnt signaling and subsequent organizer formation on the future dorsal side of the embryo. Although roles of Wnt signaling during organizer formation have been studied extensively, it is unclear how the Wnt pathway is asymmetrically activated. In Xenopus and zebrafish, the Wnt pathway is triggered by dorsal determinants, which are translocated from the vegetal pole to the future dorsal side of the embryo shortly after fertilization. The transport of dorsal determinants requires a unique microtubule network formed in the vegetal cortex shortly after fertilization. However, molecular mechanisms governing the formation of vegetal cortical microtubule arrays are not fully understood. Here we report that Dead-End 1 (Dnd1), an RNA-binding protein required for primordial germ cell development during later stages of embryogenesis, is essential for Xenopus axis specification. We show that knockdown of maternal Dnd1 specifically interferes with the formation of vegetal cortical microtubules. This, in turn, impairs translocation of dorsal determinants, the initiation of Wnt signaling, organizer formation, and ultimately results in ventralized embryos. Furthermore, we found that Dnd1 binds to a uridine-rich sequence in the 3′-UTR of trim36, a vegetally localized maternal RNA essential for vegetal cortical microtubule assembly. Dnd1 anchors trim36 to the vegetal cortex in the egg, promoting high concentrations of Trim36 protein there. Our work thus demonstrates a novel and surprising function for Dnd1 during early development and provides an important link between Dnd1, mRNA localization, the microtubule cytoskeleton and axis specification.

Original languageEnglish
Pages (from-to)2334-2344
Number of pages11
JournalDevelopment (Cambridge)
Volume140
Issue number11
DOIs
StatePublished - Jun 1 2012

Fingerprint

Xenopus
Microtubules
Mothers
Wnt Signaling Pathway
Embryonic Structures
Fertilization
RNA-Binding Proteins
Uridine
3' Untranslated Regions
Zebrafish
Cytoskeleton
Germ Cells
Embryonic Development
Ovum
Vertebrates
RNA
Messenger RNA
Proteins

Keywords

  • Axis specification
  • Cortical rotation
  • Dnd1
  • Microtubules
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Mei, W., Jin, Z., Lai, F., Schwend, T., Houston, D. W., King, M. L. L., & Yang, J. (2012). Maternal Dead-End1 is required for vegetal cortical microtubule assembly during Xenopus axis specification. Development (Cambridge), 140(11), 2334-2344. https://doi.org/10.1242/dev.094748

Maternal Dead-End1 is required for vegetal cortical microtubule assembly during Xenopus axis specification. / Mei, Wenyan; Jin, Zhigang; Lai, Fangfang; Schwend, Tyler; Houston, Douglas W.; King, Mary Lou L; Yang, Jing.

In: Development (Cambridge), Vol. 140, No. 11, 01.06.2012, p. 2334-2344.

Research output: Contribution to journalArticle

Mei, W, Jin, Z, Lai, F, Schwend, T, Houston, DW, King, MLL & Yang, J 2012, 'Maternal Dead-End1 is required for vegetal cortical microtubule assembly during Xenopus axis specification', Development (Cambridge), vol. 140, no. 11, pp. 2334-2344. https://doi.org/10.1242/dev.094748
Mei, Wenyan ; Jin, Zhigang ; Lai, Fangfang ; Schwend, Tyler ; Houston, Douglas W. ; King, Mary Lou L ; Yang, Jing. / Maternal Dead-End1 is required for vegetal cortical microtubule assembly during Xenopus axis specification. In: Development (Cambridge). 2012 ; Vol. 140, No. 11. pp. 2334-2344.
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