Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex

Tristan Aguero, Zhigang Jin, Sandip Chorghade, Auinash Kalsotra, Mary Lou L King, Jing Yang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.

Original languageEnglish (US)
Pages (from-to)3755-3765
Number of pages11
JournalDevelopment (Cambridge)
Volume144
Issue number20
DOIs
StatePublished - Oct 15 2017

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Germ Cells
Mothers
Fertilization
Embryonic Structures
Eukaryotic Initiation Factors
RNA-Binding Proteins
Infertility
Oocytes
Vertebrates
Genome
Proteins

Keywords

  • Dnd1
  • Germline development
  • Nanos
  • Translation regulation
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Aguero, T., Jin, Z., Chorghade, S., Kalsotra, A., King, M. L. L., & Yang, J. (2017). Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex. Development (Cambridge), 144(20), 3755-3765. https://doi.org/10.1242/dev.152611

Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex. / Aguero, Tristan; Jin, Zhigang; Chorghade, Sandip; Kalsotra, Auinash; King, Mary Lou L; Yang, Jing.

In: Development (Cambridge), Vol. 144, No. 20, 15.10.2017, p. 3755-3765.

Research output: Contribution to journalArticle

Aguero, T, Jin, Z, Chorghade, S, Kalsotra, A, King, MLL & Yang, J 2017, 'Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex', Development (Cambridge), vol. 144, no. 20, pp. 3755-3765. https://doi.org/10.1242/dev.152611
Aguero T, Jin Z, Chorghade S, Kalsotra A, King MLL, Yang J. Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex. Development (Cambridge). 2017 Oct 15;144(20):3755-3765. https://doi.org/10.1242/dev.152611
Aguero, Tristan ; Jin, Zhigang ; Chorghade, Sandip ; Kalsotra, Auinash ; King, Mary Lou L ; Yang, Jing. / Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex. In: Development (Cambridge). 2017 ; Vol. 144, No. 20. pp. 3755-3765.
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