Mast cells express novel functional IL-15 receptor α isoforms

Elena Bulanova, Vadim Budagian, Zane Orinska, Hans Krause, Ralf Paus, Silvia Bulfone-Paus

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Mast cells previously have been reported to be regulated by IL-15 and to express a distinct IL-15R, termed IL-15RX. To further examine IL-15 binding and signaling in mast cells, we have studied the nature of the IL-15R and some of its biological activities in these cells. In this study, we report the existence of three novel isoforms of the IL-15Rα chain in murine bone marrow-derived mast cells as a result of an alternative exon-splicing mechanism within the IL-15Rα gene. These correspond to new mRNA transcripts lacking exon 4; exons 3 and 4; or exons 3, 4, and 5 (IL-15RαΔ4, IL-15RαΔ3,4, IL-15RαΔ3,4,5). After transient transfection in COS-7 cells, all IL-15Rα isoforms associate with the Golgi apparatus, the endoplasmic reticulum, the perinuclear space, and the cell membrane. Analysis of glycosylation pattern demonstrates the usage of a single N-glycosylation site, while no O-glycosylation is observed. Importantly, IL-15 binds with high affinity to, and promotes the survival of, murine BA/F3 cells stably transfected with the IL-15Rα isoforms. Furthermore, we report that signaling mediated by IL-15 binding to the newly identified IL-15Rα isoforms involves the phosphorylation of STAT3, STAT5, STAT6, Janus kinase 2, and Syk kinase. Taken together, our data indicate that murine mast cells express novel, fully functional IL-15Rα isoforms, which can explain the selective regulatory effects of IL-15 on these cells.

Original languageEnglish (US)
Pages (from-to)5045-5055
Number of pages11
JournalJournal of Immunology
Volume170
Issue number10
StatePublished - May 15 2003
Externally publishedYes

Fingerprint

Interleukin-15 Receptors
Interleukin-15
Mast Cells
Protein Isoforms
Exons
Glycosylation
Interleukin-4
Janus Kinase 2
Interleukin-3
COS Cells
Interleukin-5
Alternative Splicing
Golgi Apparatus
Endoplasmic Reticulum
Transfection
Bone Marrow
Phosphorylation
Cell Membrane
Messenger RNA
Genes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Bulanova, E., Budagian, V., Orinska, Z., Krause, H., Paus, R., & Bulfone-Paus, S. (2003). Mast cells express novel functional IL-15 receptor α isoforms. Journal of Immunology, 170(10), 5045-5055.

Mast cells express novel functional IL-15 receptor α isoforms. / Bulanova, Elena; Budagian, Vadim; Orinska, Zane; Krause, Hans; Paus, Ralf; Bulfone-Paus, Silvia.

In: Journal of Immunology, Vol. 170, No. 10, 15.05.2003, p. 5045-5055.

Research output: Contribution to journalArticle

Bulanova, E, Budagian, V, Orinska, Z, Krause, H, Paus, R & Bulfone-Paus, S 2003, 'Mast cells express novel functional IL-15 receptor α isoforms', Journal of Immunology, vol. 170, no. 10, pp. 5045-5055.
Bulanova E, Budagian V, Orinska Z, Krause H, Paus R, Bulfone-Paus S. Mast cells express novel functional IL-15 receptor α isoforms. Journal of Immunology. 2003 May 15;170(10):5045-5055.
Bulanova, Elena ; Budagian, Vadim ; Orinska, Zane ; Krause, Hans ; Paus, Ralf ; Bulfone-Paus, Silvia. / Mast cells express novel functional IL-15 receptor α isoforms. In: Journal of Immunology. 2003 ; Vol. 170, No. 10. pp. 5045-5055.
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