TY - JOUR
T1 - Mast Cell-Mediated Antigen Presentation Regulates CD8+ T Cell Effector Functions
AU - Stelekati, Erietta
AU - Bahri, Rajia
AU - D'Orlando, Orietta
AU - Orinska, Zane
AU - Mittrücker, Hans Willi
AU - Langenhaun, Rabea
AU - Glatzel, Markus
AU - Bollinger, Annalena
AU - Paus, Ralf
AU - Bulfone-Paus, Silvia
N1 - Funding Information:
We thank M-L. Helms, G. Rode, and K. Westphal for excellent technical support, F. Mirghomizadeh for help with primer design, F. Winau for providing the B2m −/− mice, and H. Shen for providing recombinant L . monocytogenes-OVA. This work was funded in part by grants from the Deutsche Forschungsgemeinschaft (DFG) to S.B.P. (SFB/TR 22, A14).
PY - 2009/10/16
Y1 - 2009/10/16
N2 - The characteristics, importance, and molecular requirements for interactions between mast cells (MCs) and CD8+ T cells have not been elucidated. Here, we demonstrated that MCs induced antigen-specific CD8+ T cell activation and proliferation. This process required direct cell contact and MHC class I-dependent antigen cross-presentation by MCs and induced the secretion of interleukin-2, interferon-γ, and macrophage inflammatory protein-1α by CD8+ T cells. MCs regulated antigen-specific CD8+ T cell cytotoxicity by increasing granzyme B expression and by promoting CD8+ T cell degranulation. Because MCs also upregulated their expression of costimulatory molecules (4-1BB) and released osteopontin upon direct T cell contact, MC-T cell interactions probably are bidirectional. In vivo, adoptive transfer of antigen-pulsed MCs induced MHC class I-dependent, antigen-specific CD8+ T cell proliferation, and MCs regulated CD8+ T cell-specific priming in experimental autoimmune encephalomyelitis. Thus, MCs are important players in antigen-specific regulation of CD8+ T cells.
AB - The characteristics, importance, and molecular requirements for interactions between mast cells (MCs) and CD8+ T cells have not been elucidated. Here, we demonstrated that MCs induced antigen-specific CD8+ T cell activation and proliferation. This process required direct cell contact and MHC class I-dependent antigen cross-presentation by MCs and induced the secretion of interleukin-2, interferon-γ, and macrophage inflammatory protein-1α by CD8+ T cells. MCs regulated antigen-specific CD8+ T cell cytotoxicity by increasing granzyme B expression and by promoting CD8+ T cell degranulation. Because MCs also upregulated their expression of costimulatory molecules (4-1BB) and released osteopontin upon direct T cell contact, MC-T cell interactions probably are bidirectional. In vivo, adoptive transfer of antigen-pulsed MCs induced MHC class I-dependent, antigen-specific CD8+ T cell proliferation, and MCs regulated CD8+ T cell-specific priming in experimental autoimmune encephalomyelitis. Thus, MCs are important players in antigen-specific regulation of CD8+ T cells.
KW - CELLIMMUNO
KW - MOLIMMUNO
UR - http://www.scopus.com/inward/record.url?scp=70349756896&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349756896&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2009.08.022
DO - 10.1016/j.immuni.2009.08.022
M3 - Article
C2 - 19818652
AN - SCOPUS:70349756896
VL - 31
SP - 665
EP - 676
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 4
ER -