Mast Cell-Mediated Antigen Presentation Regulates CD8+ T Cell Effector Functions

Erietta Stelekati, Rajia Bahri, Orietta D'Orlando, Zane Orinska, Hans Willi Mittrücker, Rabea Langenhaun, Markus Glatzel, Annalena Bollinger, Ralf Paus, Silvia Bulfone-Paus

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The characteristics, importance, and molecular requirements for interactions between mast cells (MCs) and CD8+ T cells have not been elucidated. Here, we demonstrated that MCs induced antigen-specific CD8+ T cell activation and proliferation. This process required direct cell contact and MHC class I-dependent antigen cross-presentation by MCs and induced the secretion of interleukin-2, interferon-γ, and macrophage inflammatory protein-1α by CD8+ T cells. MCs regulated antigen-specific CD8+ T cell cytotoxicity by increasing granzyme B expression and by promoting CD8+ T cell degranulation. Because MCs also upregulated their expression of costimulatory molecules (4-1BB) and released osteopontin upon direct T cell contact, MC-T cell interactions probably are bidirectional. In vivo, adoptive transfer of antigen-pulsed MCs induced MHC class I-dependent, antigen-specific CD8+ T cell proliferation, and MCs regulated CD8+ T cell-specific priming in experimental autoimmune encephalomyelitis. Thus, MCs are important players in antigen-specific regulation of CD8+ T cells.

Original languageEnglish (US)
Pages (from-to)665-676
Number of pages12
JournalImmunity
Volume31
Issue number4
DOIs
StatePublished - Oct 16 2009
Externally publishedYes

Fingerprint

Antigen Presentation
Mast Cells
T-Lymphocytes
CD8 Antigens
Histocompatibility Antigens Class I
Cross-Priming
Macrophage Inflammatory Proteins
Antigens
Granzymes
Osteopontin
Autoimmune Experimental Encephalomyelitis
Adoptive Transfer
Cell Communication
Interferons
Interleukin-2

Keywords

  • CELLIMMUNO
  • MOLIMMUNO

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Stelekati, E., Bahri, R., D'Orlando, O., Orinska, Z., Mittrücker, H. W., Langenhaun, R., ... Bulfone-Paus, S. (2009). Mast Cell-Mediated Antigen Presentation Regulates CD8+ T Cell Effector Functions. Immunity, 31(4), 665-676. https://doi.org/10.1016/j.immuni.2009.08.022

Mast Cell-Mediated Antigen Presentation Regulates CD8+ T Cell Effector Functions. / Stelekati, Erietta; Bahri, Rajia; D'Orlando, Orietta; Orinska, Zane; Mittrücker, Hans Willi; Langenhaun, Rabea; Glatzel, Markus; Bollinger, Annalena; Paus, Ralf; Bulfone-Paus, Silvia.

In: Immunity, Vol. 31, No. 4, 16.10.2009, p. 665-676.

Research output: Contribution to journalArticle

Stelekati, E, Bahri, R, D'Orlando, O, Orinska, Z, Mittrücker, HW, Langenhaun, R, Glatzel, M, Bollinger, A, Paus, R & Bulfone-Paus, S 2009, 'Mast Cell-Mediated Antigen Presentation Regulates CD8+ T Cell Effector Functions', Immunity, vol. 31, no. 4, pp. 665-676. https://doi.org/10.1016/j.immuni.2009.08.022
Stelekati E, Bahri R, D'Orlando O, Orinska Z, Mittrücker HW, Langenhaun R et al. Mast Cell-Mediated Antigen Presentation Regulates CD8+ T Cell Effector Functions. Immunity. 2009 Oct 16;31(4):665-676. https://doi.org/10.1016/j.immuni.2009.08.022
Stelekati, Erietta ; Bahri, Rajia ; D'Orlando, Orietta ; Orinska, Zane ; Mittrücker, Hans Willi ; Langenhaun, Rabea ; Glatzel, Markus ; Bollinger, Annalena ; Paus, Ralf ; Bulfone-Paus, Silvia. / Mast Cell-Mediated Antigen Presentation Regulates CD8+ T Cell Effector Functions. In: Immunity. 2009 ; Vol. 31, No. 4. pp. 665-676.
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