Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene

Suzy D C Bianco, Ji Bin Peng, Hitomi Takanaga, Yoshiro Suzuki, Alessandra Crescenzi, Claudine H. Kos, Liyan Zhuang, Michael R. Freeman, Cecilia H A Gouveia, Jiangping Wu, Hongyu Luo, Theodora Mauro, Edward M. Brown, Matthias A. Hediger

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

We report the phenotype of mice with targeted disruption of the Trpv6 (Trpv6 KO) epithelial calcium channel. The mice exhibit disordered Ca 2+ homeostasis, including defective intestinal Ca2+ absorption, increased urinary Ca2+ excretion, decreased BMD, deficient weight gain, and reduced fertility. Although our Trpv6 KO affects the closely adjacent EphB6 gene, the phenotype reported here is not related to EphB6 dysfunction. Introduction: The mechanisms underlying intestinal Ca2+ absorption are crucial for overall Ca2+ homeostasis, because diet is the only source of all new Ca2+ in the body. Trpv6 encodes a Ca 2+-permeable cation channel responsible for vitamin D-dependent intestinal Ca2+ absorption. Trpv6 is expressed in the intestine and also in the skin, placenta, kidney, and exocrine organs. Materials and Methods: To determine the in vivo function of TRPV6, we generated mice with targeted disruption of the Trpv6 (Trpv6 KO) gene. Results: Trpv6 KO mice are viable but exhibit disordered Ca2+ homeostasis, including a 60% decrease in intestinal Ca2+ absorption, deficient weight gain, decreased BMD, and reduced fertility. When kept on a regular (1% Ca2+) diet, Trpv6 KO mice have deficient intestinal Ca2+ absorption, despite elevated levels of serum PTH (3.8-fold) and 1,25-dihydroxyvitamin D (2.4-fold). They also have decreased urinary osmolality and increased Ca2+ excretion. Their serum Ca2+ is normal, but when challenged with a low (0.25%) Ca2+ diet, Trpv6 KO mice fail to further increase serum PTH and vitamin D, ultimately developing hypocalcemia. Trpv6 KO mice have normal urinary deoxypyridinoline excretion, although exhibiting a 9.3% reduction in femoral mineral density at 2 months of age, which is not restored by treatment for 1 month with a high (2%) Ca2+ "rescue" diet. In addition to their deranged Ca2+ homeostasis, the skin of Trpv6 KO mice has fewer and thinner layers of stratum corneum, decreased total Ca2+ content, and loss of the normal Ca2+ gradient. Twenty percent of all Trpv6 KO animals develop alopecia and dermatitis. Conclusions: Trpv6 KO mice exhibit an array of abnormalities in multiple tissues/organs. At least some of these are caused by tissue-specific mechanisms. In addition, the kidneys and bones of Trpv6 KO mice do not respond to their elevated levels of PTH and 1,25-dihydroxyvitamin D. These data indicate that the TRPV6 channel plays an important role in Ca2+ homeostasis and in other tissues not directly involved in this process.

Original languageEnglish
Pages (from-to)274-285
Number of pages12
JournalJournal of Bone and Mineral Research
Volume22
Issue number2
DOIs
StatePublished - Feb 1 2007

Fingerprint

Calcium Channels
Homeostasis
Calcium
Intestinal Absorption
Genes
Diet
Vitamin D
Weight Gain
Fertility
Serum
Multiple Abnormalities
Phenotype
Kidney
Skin
Hypocalcemia
Alopecia
Dermatitis
Thigh
Osmolar Concentration
Cornea

Keywords

  • Alopecia
  • Intestinal calcium absorption
  • Renal excretion
  • TRPV6
  • Vitamin D

ASJC Scopus subject areas

  • Surgery

Cite this

Bianco, S. D. C., Peng, J. B., Takanaga, H., Suzuki, Y., Crescenzi, A., Kos, C. H., ... Hediger, M. A. (2007). Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene. Journal of Bone and Mineral Research, 22(2), 274-285. https://doi.org/10.1359/jbmr.061110

Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene. / Bianco, Suzy D C; Peng, Ji Bin; Takanaga, Hitomi; Suzuki, Yoshiro; Crescenzi, Alessandra; Kos, Claudine H.; Zhuang, Liyan; Freeman, Michael R.; Gouveia, Cecilia H A; Wu, Jiangping; Luo, Hongyu; Mauro, Theodora; Brown, Edward M.; Hediger, Matthias A.

In: Journal of Bone and Mineral Research, Vol. 22, No. 2, 01.02.2007, p. 274-285.

Research output: Contribution to journalArticle

Bianco, SDC, Peng, JB, Takanaga, H, Suzuki, Y, Crescenzi, A, Kos, CH, Zhuang, L, Freeman, MR, Gouveia, CHA, Wu, J, Luo, H, Mauro, T, Brown, EM & Hediger, MA 2007, 'Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene', Journal of Bone and Mineral Research, vol. 22, no. 2, pp. 274-285. https://doi.org/10.1359/jbmr.061110
Bianco, Suzy D C ; Peng, Ji Bin ; Takanaga, Hitomi ; Suzuki, Yoshiro ; Crescenzi, Alessandra ; Kos, Claudine H. ; Zhuang, Liyan ; Freeman, Michael R. ; Gouveia, Cecilia H A ; Wu, Jiangping ; Luo, Hongyu ; Mauro, Theodora ; Brown, Edward M. ; Hediger, Matthias A. / Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene. In: Journal of Bone and Mineral Research. 2007 ; Vol. 22, No. 2. pp. 274-285.
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abstract = "We report the phenotype of mice with targeted disruption of the Trpv6 (Trpv6 KO) epithelial calcium channel. The mice exhibit disordered Ca 2+ homeostasis, including defective intestinal Ca2+ absorption, increased urinary Ca2+ excretion, decreased BMD, deficient weight gain, and reduced fertility. Although our Trpv6 KO affects the closely adjacent EphB6 gene, the phenotype reported here is not related to EphB6 dysfunction. Introduction: The mechanisms underlying intestinal Ca2+ absorption are crucial for overall Ca2+ homeostasis, because diet is the only source of all new Ca2+ in the body. Trpv6 encodes a Ca 2+-permeable cation channel responsible for vitamin D-dependent intestinal Ca2+ absorption. Trpv6 is expressed in the intestine and also in the skin, placenta, kidney, and exocrine organs. Materials and Methods: To determine the in vivo function of TRPV6, we generated mice with targeted disruption of the Trpv6 (Trpv6 KO) gene. Results: Trpv6 KO mice are viable but exhibit disordered Ca2+ homeostasis, including a 60{\%} decrease in intestinal Ca2+ absorption, deficient weight gain, decreased BMD, and reduced fertility. When kept on a regular (1{\%} Ca2+) diet, Trpv6 KO mice have deficient intestinal Ca2+ absorption, despite elevated levels of serum PTH (3.8-fold) and 1,25-dihydroxyvitamin D (2.4-fold). They also have decreased urinary osmolality and increased Ca2+ excretion. Their serum Ca2+ is normal, but when challenged with a low (0.25{\%}) Ca2+ diet, Trpv6 KO mice fail to further increase serum PTH and vitamin D, ultimately developing hypocalcemia. Trpv6 KO mice have normal urinary deoxypyridinoline excretion, although exhibiting a 9.3{\%} reduction in femoral mineral density at 2 months of age, which is not restored by treatment for 1 month with a high (2{\%}) Ca2+ {"}rescue{"} diet. In addition to their deranged Ca2+ homeostasis, the skin of Trpv6 KO mice has fewer and thinner layers of stratum corneum, decreased total Ca2+ content, and loss of the normal Ca2+ gradient. Twenty percent of all Trpv6 KO animals develop alopecia and dermatitis. Conclusions: Trpv6 KO mice exhibit an array of abnormalities in multiple tissues/organs. At least some of these are caused by tissue-specific mechanisms. In addition, the kidneys and bones of Trpv6 KO mice do not respond to their elevated levels of PTH and 1,25-dihydroxyvitamin D. These data indicate that the TRPV6 channel plays an important role in Ca2+ homeostasis and in other tissues not directly involved in this process.",
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T1 - Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene

AU - Bianco, Suzy D C

AU - Peng, Ji Bin

AU - Takanaga, Hitomi

AU - Suzuki, Yoshiro

AU - Crescenzi, Alessandra

AU - Kos, Claudine H.

AU - Zhuang, Liyan

AU - Freeman, Michael R.

AU - Gouveia, Cecilia H A

AU - Wu, Jiangping

AU - Luo, Hongyu

AU - Mauro, Theodora

AU - Brown, Edward M.

AU - Hediger, Matthias A.

PY - 2007/2/1

Y1 - 2007/2/1

N2 - We report the phenotype of mice with targeted disruption of the Trpv6 (Trpv6 KO) epithelial calcium channel. The mice exhibit disordered Ca 2+ homeostasis, including defective intestinal Ca2+ absorption, increased urinary Ca2+ excretion, decreased BMD, deficient weight gain, and reduced fertility. Although our Trpv6 KO affects the closely adjacent EphB6 gene, the phenotype reported here is not related to EphB6 dysfunction. Introduction: The mechanisms underlying intestinal Ca2+ absorption are crucial for overall Ca2+ homeostasis, because diet is the only source of all new Ca2+ in the body. Trpv6 encodes a Ca 2+-permeable cation channel responsible for vitamin D-dependent intestinal Ca2+ absorption. Trpv6 is expressed in the intestine and also in the skin, placenta, kidney, and exocrine organs. Materials and Methods: To determine the in vivo function of TRPV6, we generated mice with targeted disruption of the Trpv6 (Trpv6 KO) gene. Results: Trpv6 KO mice are viable but exhibit disordered Ca2+ homeostasis, including a 60% decrease in intestinal Ca2+ absorption, deficient weight gain, decreased BMD, and reduced fertility. When kept on a regular (1% Ca2+) diet, Trpv6 KO mice have deficient intestinal Ca2+ absorption, despite elevated levels of serum PTH (3.8-fold) and 1,25-dihydroxyvitamin D (2.4-fold). They also have decreased urinary osmolality and increased Ca2+ excretion. Their serum Ca2+ is normal, but when challenged with a low (0.25%) Ca2+ diet, Trpv6 KO mice fail to further increase serum PTH and vitamin D, ultimately developing hypocalcemia. Trpv6 KO mice have normal urinary deoxypyridinoline excretion, although exhibiting a 9.3% reduction in femoral mineral density at 2 months of age, which is not restored by treatment for 1 month with a high (2%) Ca2+ "rescue" diet. In addition to their deranged Ca2+ homeostasis, the skin of Trpv6 KO mice has fewer and thinner layers of stratum corneum, decreased total Ca2+ content, and loss of the normal Ca2+ gradient. Twenty percent of all Trpv6 KO animals develop alopecia and dermatitis. Conclusions: Trpv6 KO mice exhibit an array of abnormalities in multiple tissues/organs. At least some of these are caused by tissue-specific mechanisms. In addition, the kidneys and bones of Trpv6 KO mice do not respond to their elevated levels of PTH and 1,25-dihydroxyvitamin D. These data indicate that the TRPV6 channel plays an important role in Ca2+ homeostasis and in other tissues not directly involved in this process.

AB - We report the phenotype of mice with targeted disruption of the Trpv6 (Trpv6 KO) epithelial calcium channel. The mice exhibit disordered Ca 2+ homeostasis, including defective intestinal Ca2+ absorption, increased urinary Ca2+ excretion, decreased BMD, deficient weight gain, and reduced fertility. Although our Trpv6 KO affects the closely adjacent EphB6 gene, the phenotype reported here is not related to EphB6 dysfunction. Introduction: The mechanisms underlying intestinal Ca2+ absorption are crucial for overall Ca2+ homeostasis, because diet is the only source of all new Ca2+ in the body. Trpv6 encodes a Ca 2+-permeable cation channel responsible for vitamin D-dependent intestinal Ca2+ absorption. Trpv6 is expressed in the intestine and also in the skin, placenta, kidney, and exocrine organs. Materials and Methods: To determine the in vivo function of TRPV6, we generated mice with targeted disruption of the Trpv6 (Trpv6 KO) gene. Results: Trpv6 KO mice are viable but exhibit disordered Ca2+ homeostasis, including a 60% decrease in intestinal Ca2+ absorption, deficient weight gain, decreased BMD, and reduced fertility. When kept on a regular (1% Ca2+) diet, Trpv6 KO mice have deficient intestinal Ca2+ absorption, despite elevated levels of serum PTH (3.8-fold) and 1,25-dihydroxyvitamin D (2.4-fold). They also have decreased urinary osmolality and increased Ca2+ excretion. Their serum Ca2+ is normal, but when challenged with a low (0.25%) Ca2+ diet, Trpv6 KO mice fail to further increase serum PTH and vitamin D, ultimately developing hypocalcemia. Trpv6 KO mice have normal urinary deoxypyridinoline excretion, although exhibiting a 9.3% reduction in femoral mineral density at 2 months of age, which is not restored by treatment for 1 month with a high (2%) Ca2+ "rescue" diet. In addition to their deranged Ca2+ homeostasis, the skin of Trpv6 KO mice has fewer and thinner layers of stratum corneum, decreased total Ca2+ content, and loss of the normal Ca2+ gradient. Twenty percent of all Trpv6 KO animals develop alopecia and dermatitis. Conclusions: Trpv6 KO mice exhibit an array of abnormalities in multiple tissues/organs. At least some of these are caused by tissue-specific mechanisms. In addition, the kidneys and bones of Trpv6 KO mice do not respond to their elevated levels of PTH and 1,25-dihydroxyvitamin D. These data indicate that the TRPV6 channel plays an important role in Ca2+ homeostasis and in other tissues not directly involved in this process.

KW - Alopecia

KW - Intestinal calcium absorption

KW - Renal excretion

KW - TRPV6

KW - Vitamin D

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