Manganese oxide particles as cytoprotective, oxygen generating agents

Mohammad Hossein Tootoonchi, Mazdak Hashempour, Patricia L. Blackwelder, Christopher Fraker

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cell culture and cellular transplant therapies are adversely affected by oxidative species and radicals. Herein, we present the production of bioactive manganese oxide nanoparticles for the purpose of radical scavenging and cytoprotection. Manganese comprises the core active structure of somatic enzymes that perform the same function, in vivo. Formulated nanoparticles were characterized structurally and surveyed for maximal activity (superoxide scavenging, hydrogen peroxide scavenging with resultant oxygen generation) and minimal cytotoxicity (48-h direct exposure to titrated manganese oxide concentrations). Cytoprotective capacity was tested using cell exposure to hydrogen peroxide in the presence or absence of the nanoparticles. Several ideal compounds were manufactured and utilized that showed complete disproportionation of superoxide produced by the xanthine/xanthine oxidase reaction. Further, the nanoparticles showed catalase–like activity by completely converting hydrogen peroxide into the corresponding concentration of oxygen. Finally, the particles protected cells (murine β-cell insulinoma) against insult from hydrogen peroxide exposure. Based on these observed properties, these particles could be utilized to combat oxidative stress and inflammatory response in a variety of cell therapy applications. Statement of Significance Maintaining viability once cells have been removed from their physiological niche, e.g. culture and transplant, demands proper control of critical variables such as oxygenation and removal of harmful substances e.g. reactive oxygen species. Limited catalysts can transform reactive oxygen species into molecular oxygen and, thereby, have the potential to maintain cell viability and function. Among these are manganese oxide particles which are the subject of this study.

Original languageEnglish (US)
Pages (from-to)327-337
Number of pages11
JournalActa Biomaterialia
Volume59
DOIs
StatePublished - Sep 1 2017

Fingerprint

Manganese oxide
Hydrogen peroxide
Nanoparticles
Hydrogen Peroxide
Scavenging
Oxygen
Transplants
Superoxides
Reactive Oxygen Species
Cell Survival
Insulinoma
Xanthine
Oxidative stress
Oxygenation
Cytoprotection
Molecular oxygen
Xanthine Oxidase
Cytotoxicity
Manganese
Cell- and Tissue-Based Therapy

Keywords

  • Cell culture
  • Cell encapsulation
  • Cytoprotection
  • In vitro oxygen generation
  • Nanoparticles
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Manganese oxide particles as cytoprotective, oxygen generating agents. / Tootoonchi, Mohammad Hossein; Hashempour, Mazdak; Blackwelder, Patricia L.; Fraker, Christopher.

In: Acta Biomaterialia, Vol. 59, 01.09.2017, p. 327-337.

Research output: Contribution to journalArticle

Tootoonchi, Mohammad Hossein ; Hashempour, Mazdak ; Blackwelder, Patricia L. ; Fraker, Christopher. / Manganese oxide particles as cytoprotective, oxygen generating agents. In: Acta Biomaterialia. 2017 ; Vol. 59. pp. 327-337.
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