Mamu-I: A novel primate MHC class I B-related locus with unusually low variability

Julie A. Urvater, Nel Otting, Jamie H. Loehrke, Richard Rudersdorf, Igor I. Slukvin, Marian S. Piekarczyk, Thaddeus G. Golos, Austin L. Hughes, Ronald E. Bontrop, David Watkins

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The rhesus macaque is an important animal model for several human diseases and organ transplantation. Therefore, definition of the MHC of this species is crucial to the development of these models. Unfortunately, unlike humans, lymphocytes from a single rhesus macaque express up to 12 different MHC class I cDNAs. From which locus these various alleles are derived is unclear. In our attempts to define the MHC class I loci of the rhesus macaque, we have identified an unusual MHC class I locus, Mamu-I. We isolated 26 I locus alleles from three different macaque species but not from three other Cercopithecine genera, suggesting that the I locus is the result of a recent duplication of the B locus occurring after the divergence of macaques from the ancestor of the other extant Cercopithecine genera, Mamu-I mRNA transcripts were detected in all tissues examined and Mamu-I protein was produced in rhesus B lymphoblastoid cell lines. Furthermore, Mamu-I protein was detected by flow cytometry on the surface of human 721.221 cells transfected with Mamu-I. In contrast to the polymorphism present at this locus, there is unusually low sequence variability, with the mean number of nucleotide differences between alleles being only 3.6 nt. Therefore, Mamu-I is less variable than any other polymorphic MHC class I locus described to date. Additionally, no evidence for positive selection on the peptide binding region was observed. Together, these results suggest that Mamu-I is an MHC class I locus in primates that has features of both classical and nonclassical loci.

Original languageEnglish
Pages (from-to)1386-1398
Number of pages13
JournalJournal of Immunology
Volume164
Issue number3
StatePublished - Feb 1 2000
Externally publishedYes

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Macaca mulatta
Primates
Alleles
Macaca
Organ Transplantation
Flow Cytometry
Proteins
Nucleotides
Animal Models
Complementary DNA
Lymphocytes
Cell Line
Messenger RNA
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Urvater, J. A., Otting, N., Loehrke, J. H., Rudersdorf, R., Slukvin, I. I., Piekarczyk, M. S., ... Watkins, D. (2000). Mamu-I: A novel primate MHC class I B-related locus with unusually low variability. Journal of Immunology, 164(3), 1386-1398.

Mamu-I : A novel primate MHC class I B-related locus with unusually low variability. / Urvater, Julie A.; Otting, Nel; Loehrke, Jamie H.; Rudersdorf, Richard; Slukvin, Igor I.; Piekarczyk, Marian S.; Golos, Thaddeus G.; Hughes, Austin L.; Bontrop, Ronald E.; Watkins, David.

In: Journal of Immunology, Vol. 164, No. 3, 01.02.2000, p. 1386-1398.

Research output: Contribution to journalArticle

Urvater, JA, Otting, N, Loehrke, JH, Rudersdorf, R, Slukvin, II, Piekarczyk, MS, Golos, TG, Hughes, AL, Bontrop, RE & Watkins, D 2000, 'Mamu-I: A novel primate MHC class I B-related locus with unusually low variability', Journal of Immunology, vol. 164, no. 3, pp. 1386-1398.
Urvater JA, Otting N, Loehrke JH, Rudersdorf R, Slukvin II, Piekarczyk MS et al. Mamu-I: A novel primate MHC class I B-related locus with unusually low variability. Journal of Immunology. 2000 Feb 1;164(3):1386-1398.
Urvater, Julie A. ; Otting, Nel ; Loehrke, Jamie H. ; Rudersdorf, Richard ; Slukvin, Igor I. ; Piekarczyk, Marian S. ; Golos, Thaddeus G. ; Hughes, Austin L. ; Bontrop, Ronald E. ; Watkins, David. / Mamu-I : A novel primate MHC class I B-related locus with unusually low variability. In: Journal of Immunology. 2000 ; Vol. 164, No. 3. pp. 1386-1398.
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