Mammalian retinal pigment epithelial cells in vitro respond to the neurokines ciliary neurotrophic factor and leukemia inhibitory factor

S. K. Gupta, C. A. Jollimore, M. J. McLaren, George Inana, M. E M Kelly

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We examined whether primary cultures of rat retinal pigment epithelial (RPE) cells and RPE cells of an immortalized rat cell line, BPEI-1, would be responsive to the neurokines ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF), which are known to be potent trophic factors for neuronal cells. Primary RPE cell cultures were characterized by indirect immunofluorescence and exhibited positive immunoreactivity for RET-PE2, a monoclonal antibody that recognizes RPE cells, and for the intermediate filaments cytokeratin and vimentin. The survival of cultured RPE cells in serum-free defined medium in the presence of CNTF or LIF was investigated during a 0- to 5-day period. Both CNTF and LIF, at concentrations of 1-50 ng/mL (4-200 pM), markedly enhanced RPE cell survival. Bromodeoxyuridine labelling of RPE cells revealed an increased mitotic activity in cell cultures treated with either CNTF or LIF in comparison to untreated serum-free cultures. Increases in cell survival and proliferation after neurokine treatment were also observed with the BPEI-1 cell line. However, in comparison to the primary RPE cultures, LIF was more effective than CNTF in promoting survival of the cell line over a 5-day treatment period. These studies demonstrate that the neurokines CNTF and LIF are potent trophic factors for mammalian RPE cells in vitro and may serve as candidate therapeutic agents in degenerative conditions that affect the retina and RPE.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalBiochemistry and Cell Biology
Volume75
Issue number2
StatePublished - Dec 1 1997

Fingerprint

Ciliary Neurotrophic Factor
Leukemia Inhibitory Factor
Retinal Pigments
Epithelial Cells
Cells
Cell culture
Cell Line
Rats
Cell Survival
Cell Culture Techniques
In Vitro Techniques
Intermediate Filaments
Serum-Free Culture Media
Vimentin
Bromodeoxyuridine
Indirect Fluorescent Antibody Technique
Keratins
Labeling
Retina
Therapeutics

Keywords

  • Cell survival
  • CNTF
  • LIF
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Mammalian retinal pigment epithelial cells in vitro respond to the neurokines ciliary neurotrophic factor and leukemia inhibitory factor. / Gupta, S. K.; Jollimore, C. A.; McLaren, M. J.; Inana, George; Kelly, M. E M.

In: Biochemistry and Cell Biology, Vol. 75, No. 2, 01.12.1997, p. 119-125.

Research output: Contribution to journalArticle

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